-
Plasmid#165990PurposeSpyCatcher-fused influenza M2 ectodomain with removable HisTagDepositorInsertSpyCatcher-M2e
UseTagsHisTag and SpyCatcherExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C - AVITEV
Plasmid#74058Purposeretroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCG-D-mSpyCatcher-His6-E
Plasmid#87376PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assDepositorInsertmSpyCatcher
UseSynthetic BiologyTagsHis6 tagExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcherEQ-sfGFP
Plasmid#107421PurposeFor production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
UseTagsHis tag, StrepII, and superfolder GFPExpressionBacterialMutationmutation of glutamate 77 to glutaminePromoterTetAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIG-AVITEV- hNFATc1/aA
Plasmid#74057Purposeretroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc1, isoform alpha-A (NFATC1 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationmutation G1157A [R235Q] and the silent mutation C…PromoterpMSCV-LTRsAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-trAtCCD8(pYL736)
Plasmid#178287PurposeExpresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc1/bC - AVITEV
Plasmid#74059Purposeretroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 M C-V5-tag
Plasmid#179986PurposeMammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 M
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEt28a-H6-SUMO-SilkCatcher
Plasmid#230902PurposeBacterial expression of SilkCatcherDepositorInsertH6-SUMO-SilkCatcher
UseTagsHisTag, thrombin, SUMOExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
p2T_CAG_SpCas9PE2_Igf1-NFATC2IP_BlastR (pepPE2_3)
Plasmid#173067PurposeExpresses NI-PE2DepositorInsertIgf1-NFATC2IP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-S6Y_AroPATCHY3
Plasmid#215632PurposeFor overexpression of Gal4-DBD S6Y AroPATCHY 3DepositorInsertS6Y AroPATCHY 3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-S6Y_AroPATCHY1
Plasmid#215633PurposeFor overexpression of Gal4-DBD S6Y AroPATCHY1DepositorInsertS6Y AroPATCHY1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
sCatch-GFP-6xHIS
Plasmid#186904PurposeBacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tagDepositorInsertsfGFP-SpyCatcher003-sfCherry1-10-6xHIS
UseTags6xHis and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-ratCENP-C
Plasmid#174445Purposein vitro transcription template for rat CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAS attB V5 dDispatched WT HA
Plasmid#126411PurposeExpression of wild type dDispatched HA in Drosophila.DepositorInsertd-Dispatched1 (disp Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDJ39 - Peft-3 Cre (PATCs)
Plasmid#191381PurposeHigh efficiency CRE recombinase expression in C. elegansDepositorInsertPeft-3 Cre tbb-2 UTR
UseTagsExpressionWormMutationPromoterPeft-3Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only