Showing: 181 - 200 of 3700 results
-
Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
UseTagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
UseTagsExpressionMammalianMutationPromoterPGK/U6AvailabilityAcademic Institutions and Nonprofits only -
MmXYRS-4xM15
Plasmid#155343PurposePlasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.DepositorInsertHumanized MmXYRS/tRNAM15
UseTagsExpressionMammalianMutationPromoterCMV/U6AvailabilityAcademic Institutions and Nonprofits only -
DT044A
Plasmid#159405PurposeInducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)DepositorInsertpr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterT7/LacO promoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP FLAG
Plasmid#59760Purposeencodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_FLAG
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP OLLAS
Plasmid#59761Purposeencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_OLLAS
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Ruby2sm-Flag.WPRE.SV40
Plasmid#98925PurposeAAV expression of Ruby2 spaghetti monster FLAG constructDepositorInsertRuby2-smFP-Flag
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GFPsm-myc.WPRE.SV40
Plasmid#98926PurposeAAV expression of GFP spaghetti monster myc constructDepositorInsertGFP-smFP-myc
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40
Plasmid#98927PurposeCre dependent AAV expression of GFP spaghetti monster myc constructDepositorHas ServiceAAV1InsertGFP-smFP-myc
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailabilityAcademic Institutions and Nonprofits only -
MSGV1-1D3-28Z.1-3 mut
Plasmid#107227PurposeAnti-mouse CD19 CAR with CD3 ITAMs mutatedDepositorInsert1D3-28Z. 1-3 mut
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailabilityAcademic Institutions and Nonprofits only -
pMTgR2
Plasmid#107286PurposePlasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.DepositorInsertInterferon gamma receptor 2 (IFNGR2 Human)
UseTagsCATCATCACCATCACCATGAExpressionInsectMutationPromoterMetallothionein promoterAvailabilityAcademic Institutions and Nonprofits only -
SLC9A3R2_PDZ_2
Plasmid#103926PurposeProtein expression and purification of NHERF2 PDZ2 domainDepositorInsertSLC9A3R2 (NHERF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Plasmid#98928PurposeCre dependent AAV expression of Ruby2 spaghetti monster FLAG constructDepositorHas ServiceAAV1InsertRuby2-smFP-Flag
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NC-CRISPRi (Gen3)
Plasmid#73499PurposeConstitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertdCas9-KRAB
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pCMV-αIFNα-ib
Plasmid#110798PurposeExpression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cellsDepositorInsertintrabody against mouse interferon alpha
UseTagsER retention signal (SEKDEL), ER signal peptide, …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-αIFNα-ab
Plasmid#110799PurposeExpression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cellsDepositorInsertantibody against mouse interferon alpha
UseTagsER signal peptide, His6 tag, and myc epitope tagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen1)
Plasmid#73497PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry markerDepositorInsertsdCas9-KRAB-P2A-mCherry
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen2)
Plasmid#73498PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertsdCas9-KRAB
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailabilityAcademic Institutions and Nonprofits only -
pFLAG K60A-CPTP
Plasmid#170743PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertCPTP (CPTP Human)
UseTagsFLAGExpressionMammalianMutationK60APromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 181 - 200 of 3700 results