We narrowed to 5,384 results for: kras

1. pLVUHshp53-tTR-KRAB

   Plasmid

   #11654

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+FLAG-KrascomG12D

   Plasmid

   #206844

   Purpose

   To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codons altered to most optimum based on mouse cod…

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330a dCas9-KRAB

   Plasmid

   #92361

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

   Plasmid

   #172985

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-mCherry-KRasCT

   Plasmid

   #175835

   Purpose

   Express mCherry with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with plasma membrane-targeting sequence
   

   Use

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-KRAS-G12V

   Plasmid

   #116424

   Purpose

   Lentiviral expression of KRAS G12V

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVET-tTR-KRAB

   Plasmid

   #11644

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hEF-1alpha, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVPT-tTR-KRAB

   Plasmid

   #11642

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNOC_dCas9-KRAB_sgRNA Td2

   Plasmid

   #176264

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   Kruppel associated box domain and luciferase

   Expression

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Kras (G12V)-pcw107

    Plasmid

    #64602

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2_T7-mScarlet3-KRas

    Plasmid

    #225929

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-KRas
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMVTO-KRAS-G12D

    Plasmid

    #179656

    Purpose

    KRAS active mutant (G12D mutation)

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    KRAS G12D
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

    Plasmid

    #172984

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInducer10b-EGFP-KRAS-WT

    Plasmid

    #164935

    Purpose

    Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ubc Promoter and Ubc promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX-SpiCee-Kras

    Plasmid

    #140838

    Purpose

    mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-Kras
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVPRT-tTR-KRAB

    Plasmid

    #11648

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hPrion, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TET-On

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pInducer10b-HA-KRAS G12V CO SR

    Plasmid

    #164928

    Purpose

    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

    Depositor

    Ji Luo

    Article

    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

    Insert

    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mutation

    Glycine 12 changed to valine; 9 Valine codons are…

    Promoter

    Ubc promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+FLAG-KrasnatG12D

    Plasmid

    #206842

    Purpose

    To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Aspartic Acid

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR2.1-KRAS-G12V

    Plasmid

    #129091

    Purpose

    KI KRAS G12V mutation into human KRAS locus

    Depositor

    Toshiro Sato

    Article

    Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

    Insert

    KRAS(G12V) (KRAS Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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