We narrowed to 5,806 results for: pCas

1. pCAT024

   Plasmid

   #171636

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BFP
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. M-SPcas

   Plasmid

   #48668

   Purpose

   Mammalian S. pyogenes Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Promoter

   CMV

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAM17

   Plasmid

   #86473

   Purpose

   Expresses p12 and p9

   Depositor

   Pamela Silver

   Article

   Myhrvold et al ACS Synth Biol. 2016 Aug 3.

   Insert

   p12, p9
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9_human_ADAR2_ccC

   Plasmid

   #158112

   Purpose

   deletion hADAR2

   Depositor

   Emanuela Felley-Bosco

   Article

   Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

   Insert

   ADAR2 (ADARB1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS_2paLINX

   Plasmid

   #129655

   Purpose

   2P-activatable light-inducible nuclear exporter

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paLINX
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_TWIST1

   Plasmid

   #215027

   Purpose

   Mammalian expression of human TWIST1

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   twist family bHLH transcription factor 1 (TWIST1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9_mouse_Adar1_ccB

   Plasmid

   #158122

   Purpose

   deletion mAdar1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (Adar Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9_mouse_Adar1_ccA

   Plasmid

   #158120

   Purpose

   deletion mAdar1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (Adar Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAT222

   Plasmid

   #134890

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23119

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG:EYFP

    Plasmid

    #32596

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

    Insert

    EYFP
    

    Expression

    Mammalian

    Promoter

    pCAGGs

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

12. HeFSpCas9

    Plasmid

    #92355

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAH01SpR

    Plasmid

    #128159

    Purpose

    pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCapV

    Plasmid

    #183028

    Purpose

    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28

    Depositor

    Ute Romling

    Article

    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

    Insert

    patatin-like phospholipase CapV
    

    Tags

    No

    Expression

    Bacterial

    Promoter

    pBAD promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAGM9121_pCAT1

    Plasmid

    #160918

    Purpose

    Catalase 1 (CAT1) promoter module for episomal P. pastoris promoter shuffling

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

    Insert

    Catalase 1 promoter
    

    Use

    Synthetic Biology

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. HeFm2SpCas9

    Plasmid

    #92111

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    “Highly enhanced Fidelity” mut2 SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCARSF85

    Plasmid

    #64694

    Purpose

    H6-TF-Smt3-GB1-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Tags

    CBD, HisTag, NpuDnaE-N102, Smt3, and TF

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAT221

    Plasmid

    #134889

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAD54_CAD29_H104A_pvp008

    Plasmid

    #124876

    Purpose

    Expresses hCAD His103Ala mutant in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    mutated His103 to Ala, deleted amino acids 1-3 an…

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAM35

    Plasmid

    #86475

    Purpose

    Expresses p9-6His

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p9-6His
    

    Use

    Synthetic Biology

    Tags

    6His

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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