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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,672 results for: pCas

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Showing: 181 - 200 of 2672 results

  1. VPR-ps-dSpCas9

    Plasmid
    #102855
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9, pdDronpa1.2
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS and VP64-p64-Rta transcription activator
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102855-plasmid-map-sequence-id-199006

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  7. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Publication
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80424-plasmid-map-sequence-id-151441

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  8. pX551-miniCMV-SpCas9

    Plasmid
    #107031
    Purpose
    AAV plasmid expressing Cas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107031-plasmid-map-sequence-id-208545

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  11. pMA-SpCas9-g1

    Plasmid
    #80784
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80784-plasmid-map-sequence-id-162402

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  12. pCas9-VRER_2A_GFP

    Plasmid
    #75475
    Purpose
    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control
    Depositor
    Marc Tessier-Lavigne
    Publication
    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VRER
    Use
    CRISPR
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, T1337R
    Promoter
    CAG
    Available since
    May 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75475-plasmid-map-sequence-id-142912

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  19. pMA-SpCas9-g2

    Plasmid
    #80785
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80785-plasmid-map-sequence-id-162403

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Showing: 181 - 200 of 2672 results