Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,509 results for: piggyBac

Showing: 181 - 200 of 1509 results

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PBCAG-mRFP

Plasmid

#40996

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

mRFP

Expression

Mammalian

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_BFP-3xHA

Plasmid

#254373

Purpose

Expression of BFP as a negative control in the ZSWIM8 in-cell microRNA stability assay

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

EBFP2

Tags

3xHA

Expression

Mammalian

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(F1261A)

Plasmid

#254364

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

F1261A

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(H199A)

Plasmid

#254351

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

H199A

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(mono)

Plasmid

#254354

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

Duplicate residues 222-273 within 228-229, flanke…

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(Δdimer)

Plasmid

#254355

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

Replace residues 219-275 with 3x(GGGS)

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(Y320A)

Plasmid

#254360

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

Y320A

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

MBP promoter fragment (Mbp Mouse)

Expression

Mammalian

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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PiggyBac-FLAG-mProser1-IRES-Blast

Plasmid

#226172

Purpose

Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

Insert

Proline Serine Rich protein 1 (Proser1 Mouse)

Tags

2X FLAG

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

Plasmid

#159756

Purpose

PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PBCAG-EGFR

Plasmid

#40974

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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PiggyBac CAG eGFP-PolQ-FLAG P2A-BLAST

Plasmid

#249811

Purpose

Expresses the PolQ (polymerase theta) protein fused with N-terminal eGFP and C-terminal FLAG tag

Depositor

Raphael Ceccaldi

Article

Gelot et al Nature. 2023 Sep;621(7978):415-422. doi:

Insert

Polymerase Theta (POLQ Human)

Tags

FLAG and eGFP

Expression

Mammalian

Promoter

CAG

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

Plasmid

#209085

Purpose

Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)
BirA

Expression

Mammalian

Mutation

human codon optimized

Promoter

TRE4G and hSyn

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

Plasmid

#209084

Purpose

Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
BirA

Expression

Mammalian

Mutation

human codon optimized

Promoter

hSyn

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

Plasmid

#209078

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

3K mutant: E35K, E46K, E61K

Promoter

TRE4G

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(337-343A)

Plasmid

#254361

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

Replace residues 337-343 with alanines

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(L1372A, N1373A)

Plasmid

#254362

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

L1372A, N1373A

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(T1303A, Y1304A)

Plasmid

#254363

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

T1303A, Y1304A

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(P75A, L76A)

Plasmid

#254352

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

P75A, L76A

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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piggyBac_Tet-On_HsZSWIM8(V100A, P101A)

Plasmid

#254353

Purpose

Expression of ZSWIM8 to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human ZSWIM8 (KIAA0913 Human)

Expression

Mammalian

Mutation

V100A, P101A

Promoter

TRE3G

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 181 - 200 of 1509 results

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