164,060 results

1. dSpyCas9-p300-P2A-CASANOVA

   Plasmid

   #113129

   Purpose

   Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   dCas9-p300-P2A-CASANOVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SynTagMA_pre

   Plasmid

   #119738

   Purpose

   Synaptic Tag for Mapping Activity

   Depositor

   Thomas Oertner

   Article

   Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

   Insert

   SynTagMA_pre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hsyn1

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-RPS6 (pDD1902)

   Plasmid

   #52913

   Purpose

   contains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.

   Depositor

   Dirk Dittmer

   Article

   Chen et al J Virol. 2011 Sep;85(18):9495-505. doi:

   Insert

   HAS6 (RPS6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

   Plasmid

   #177919

   Purpose

   Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-CAG-jGCaMP8m-WPRE (AAV Retrograde)

   Viral Prep

   #179255-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CAG

   Tags

   None

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

6. pMSP1E3

   Plasmid

   #20064

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1E3 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1; contains repeats of he…

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL

   Plasmid

   #218759

   Purpose

   Provides AND intersectional (Cre+Flp) expression of HA-hM4Di

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   hM4Di
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

   Plasmid

   #32739

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   TCF4E (Tcf7l2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   The N-terminal deletion form (delta N) lacks amin…

   Promoter

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 Ash2L full

   Plasmid

   #15548

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   Ash2L (ASH2L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R622H (Please see depositor comments below)

   Promoter

   Available since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Man2a1-GFP

    Plasmid

    #163649

    Purpose

    Expresses GFP tagged Man2a1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mannosidase alpha class 2A member 1 (MAN2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutation was made to introduce a XbaI rest…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

    Plasmid

    #177920

    Purpose

    Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-Squash-SAM-sensor 5-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Ii-Str_ssSBP-EGFP

    Plasmid

    #65277

    Purpose

    to synchronize transport of secretory soluble GFP from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    EGFP
    

    Use

    Tags

    IL-2 signal sequence and Streptavidin Binding Pr…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. μ2-HA-WT

    Plasmid

    #32752

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TPC2-mCherry

    Plasmid

    #135183

    Purpose

    Expresses TPC2 with C-terminus mCherry tag in mammalian cells

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pc DNA FRT TO- APPSwed/Ind

    Plasmid

    #114194

    Purpose

    Creation of a stable cell line expressing APP mutation (AD in vitro study)

    Depositor

    Aleksandra Radenovic

    Insert

    Amyloid-Precursor-Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Popular mouse model, overexpress 2 mutations: Swe…

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-Cas9-gRNA-GFP

    Plasmid

    #124770

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF1977

    Plasmid

    #144120

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX1 (TBX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBBR15.2-2H12.D11opt

    Plasmid

    #221185

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

    Plasmid

    #31357

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    NSD3 (NSD3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCHA-VRC01-scFv

    Plasmid

    #44874

    Depositor

    Dane Wittrup

    Article

    Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

    Insert

    VRC01 scFv
    

    Use

    Tags

    HA and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge