We narrowed to 4,353 results for: Abo
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Plasmid#128279PurposeMammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion proteinDepositorInserthTetR::NLS::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsEGFPExpressionMammalianPromoterCMV-D2ir promoterAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVL37 - pWOX4
Plasmid#116024Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpWOX4
UseUnspecifiedMutationT1473A by SDM to abolish Eco31I siteAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL27 - pPXY
Plasmid#116017Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpPXY
UseUnspecifiedMutationT2139A by SDM to abolish Eco31I siteAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_25-eGFP
Plasmid#107413PurposeThis plasmid contain promoter PthrC3_25, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3_25Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_7-eGFP
Plasmid#107412PurposeThis plasmid contain promoter PthrC3_7, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3_7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
nucHCB1
Plasmid#97424PurposeNuclear Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP and nuclear localization signal.DepositorInsertnucHCB1
TagsNLS and YFPExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETC-PITPbeta (splice variant 1) K61A
Plasmid#58271Purposebacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)DepositorInsertPITP beta (PITPNB Human)
TagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Start-Stop Assembly Toolkit
Plasmid Kit#1000000152PurposeStart-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.DepositorAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
Laconic/pcDNA3.1(-)
Plasmid#44238Purposea genetically-encoded sensor for lactate concentration, transport and metabolic fluxDepositorInsertLaconic (LACtate Optical Nano Indicator from CECs)
UseLactate biosensorExpressionMammalianPromoterCMVAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
ExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPRv2-sgEGFP
Plasmid#86153PurposeLentivirus carrying Cas9/CRISPR for cut in GFP (used as control)DepositorInsertEGFP
UseCRISPR and LentiviralAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-GpNLuc
Plasmid#135949PurposeExpresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertGpNLuc LumiFluor
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EGFP-IRES(Neo)R
Plasmid#158068PurposeeGFP under the TRE3G promoter, Neo resistance. For cloning GOIDepositorInserteGFP
TagseGFPExpressionMammalianPromoterTRE3GAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT(CO)-MBIS(CO, ispA)
Plasmid#35151DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5,Available SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH2
Plasmid#204230PurposeBacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).InserthMDH2 (MDH2 Human)
Tags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130A wgMDH
Plasmid#204266PurposeBacterial expression of R130A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR130AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
PECAM1-3'gRNA
Plasmid#235606PurposegRNA targeting PECAM1 3' terminal for CRISPR-Cas9-mediated knock-inDepositorAvailable SinceJune 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin/Δp120
Plasmid#45770DepositorInserthE-cadherin/Δp120 (CDH1 Human)
ExpressionMammalianMutationAmino acids 762-764 converted from EED to AAA to …PromoterCMVAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124A wgMDH
Plasmid#204257PurposeBacterial expression of R124A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
PS Intein/C1A Bid
Plasmid#242025PurposePS Intein Bid construct containing the C1A mutation in gp41-1, which abolishes its autocatalytic splicing activity.DepositorInsertPS Intein/C1A Bid
ExpressionMammalianPromoterCMVAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-VSVG
Plasmid#11915DepositorInsertVSVG
ExpressionMammalianMutation(L64F/T65S/V163A/T203H). The PAGFP also contains …Available SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
PTP1B human
Plasmid#102719Purposehuman PTP1B catalytic domain (residues 1-301) in RP1B vectorDepositorInsertProtein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196A wgMDH
Plasmid#204271PurposeBacterial expression of R196A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR196AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRVL-3
Plasmid#104581PurposeContains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functionsDepositorInsertMouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
ExpressionMammalianMutationL234A/L235E/G237A and E318A/K320A/K322APromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVenus-VSVG
Plasmid#11914DepositorInsertVSVG
ExpressionMammalianMutationF46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialPromoterPydfJ115Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationQ58LPromoterT5Available SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-PpAPOBEC1
Plasmid#138349PurposeMammalian expression plasmid for BE4-PpABOBEC1DepositorInsertBE4-PpAPOBEC1
TagsBP-NLSExpressionMammalianPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SynP_DIO_tAKARα- T391A_WPRE
Plasmid#196676PurposeAAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).DepositorInserttAKARα-T391A
UseAAV and Cre/LoxExpressionMammalianAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPL13-ECFP-SNAP
Plasmid#187967Purposeexpresses RPL13 with C-terminal tags ECFP and SNAPDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-PpAPOBEC1 R33A
Plasmid#138345PurposeMammalian expression plasmid for BE4-PpABOBEC1 R33ADepositorInsertBE4-PpAPOBEC1 R33A
UseSynthetic BiologyTagsBP-NLSMutationChange of Arg 33 to Ala.PromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTG06-Amp-FRT
Plasmid#128275PurposeDeg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124K wgMDH
Plasmid#204258PurposeBacterial expression of R124K wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124KAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124Q wgMDH
Plasmid#204259PurposeBacterial expression of R124Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124QAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130E wgMDH
Plasmid#204267PurposeBacterial expression of R130E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR130EAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-Venus
Plasmid#58328PurposeDestination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genomeDepositorInsertCad-Venus
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-PpAPOBEC1 H122A
Plasmid#138338PurposeMammalian expression plasmid for BE4-PpABOBEC1H122ADepositorInsertBE4-PpAPOBEC1 H122A
UseSynthetic BiologyTagsBP-NLSMutationChange of His122 to Ala.PromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-crScaffold_SV40-BFP
Plasmid#224860PurposecrRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6Available SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only