We narrowed to 2,015 results for: Endog

1. AAV-KrasG12A/sgKras/Cre

   Plasmid

   #99849

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-KrasG13V/sgKras/Cre

   Plasmid

   #99860

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-KrasG13S/sgKras/Cre

   Plasmid

   #99859

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-KrasG13A/sgKras/Cre

   Plasmid

   #99855

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-KrasG13R/sgKras/Cre

   Plasmid

   #99858

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

   Plasmid

   #112021

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta/alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUCM-CLYBL-NGN2-BRN3A

   Plasmid

   #165597

   Purpose

   Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

   Depositor

   Carsten Bönnemann

   Article

   Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   BRN3A (POU4F1 Human)
   EGFP
   Puromycin N-acetyl transferase
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

   Available since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

   Plasmid

   #112022

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha/beta into TRBC1 HDRDT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NYESO alpha into TRAC HDRT Source (pTR 223)

   Plasmid

   #112023

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NYESO beta into TRBC1 HDRT Source (pTR 277)

    Plasmid

    #112024

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta into TRBC1 HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RAB11A-GFP HDRT Source (pTR 143)

    Plasmid

    #112012

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-GFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RAB11A-mCherry HDRT Source (pTR 144)

    Plasmid

    #112013

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-mCherry HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CD4-C-GFP HDRT Source (pTR 152)

    Plasmid

    #112018

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-GFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CLTA-GFP HDRT Source (pTR 153)

    Plasmid

    #112016

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-GFP HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CLTA-mCherry HDRT Source (pTR 177)

    Plasmid

    #112017

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-mCherry HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BATF-GFP HDRT Source (pTR 146)

    Plasmid

    #112015

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    BATF-GFP HDRT (BATF Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RAB11A-BFP HDRT Source (pTR 145)

    Plasmid

    #112014

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-BFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CD4-C-BFP HDRT Source (pTR 176)

    Plasmid

    #112020

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-BFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CD4-C-mCherry HDRT Source (pTR 175)

    Plasmid

    #112019

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-mCherry HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19_18GZG

    Plasmid

    #117228

    Purpose

    shble flanked by an endogenous GAPDH promoter and terminator system

    Depositor

    Jackie Collier

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    shble
    

    Use

    Thraustochytrid expression

    Tags

    Expression

    Mutation

    Promoter

    GAPDH

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge