We narrowed to 5,851 results for: KRAS

Showing: 1981 - 2000 of 5851 results

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SpCas9-CBE6a-V106W

Plasmid

#215824

Purpose

Express CBE6a-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP1-10gly-Gal4DBD

Plasmid

#49438

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP1-Gal4 DNA binding domain fusion protein

Expression

Mammalian

Mutation

C98S (to increase protein solubility), Q3D

Promoter

CAG

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-RT004-LaG30VB

Plasmid

#163384

Purpose

2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

LaG30-VEEGFRtm-BFP (LaG30VB)

Use

Lentiviral

Tags

BFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBS1ClacZ

Plasmid

#55171

Purpose

lacZ-reporter vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3.0_mitoLAMA-F98

Plasmid

#130704

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDAS12124_PEAR-GFP-preedited

Plasmid

#177179

Purpose

PEAR-GFP control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRDB_161

Plasmid

#216091

Purpose

Cas9 [Sp] CRISPRi targeting CD81, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD81 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-metap2

Plasmid

#100512

Purpose

Expresses HA tagged metap2 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-RT005-HaloGPM

Plasmid

#163385

Purpose

2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM4723:TpCC_Urease

Plasmid

#85982

Purpose

Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Inserts

Cas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2

Use

CRISPR; Diatom (t. pseudonana)

Tags

NLS and YFP

Promoter

FCP and U6

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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PlepA

Plasmid

#55175

Purpose

Strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

lepA promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pPD0056_pIVTRup_dCas9-VP64

Plasmid

#242542

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

dCas9-VP64

Use

CRISPR

Mutation

D10A

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid

#73173

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Expression

Mammalian and Yeast

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDF0352 HvsCas7-11 with C-term Nes mammalian expression

Plasmid

#172516

Purpose

Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

C-term Nes HvsCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5_ALFA-nb

Plasmid

#238262

Purpose

For overexpression of ALFA-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

ALFA-nb

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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AA177

Plasmid

#215983

Purpose

Fragmid fragment: (N' terminus) Start codon only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA178

Plasmid

#216046

Purpose

Fragmid fragment: (C' terminus) Stop only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Gateway Cloning

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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