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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,103 results for: actin

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Showing: 1981 - 2000 of 6103 results

  2. MSP1594

    Plasmid
    #65775
    Purpose
    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65775-plasmid-map-sequence-id-112658

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  6. pCAG mito-mEos2

    Plasmid
    #168515
    Purpose
    Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate
    Depositor
    Franck Polleux
    Publication
    Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mito-mEos2
    Tags
    mEos2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    168515-plasmid-map-sequence-id-329879

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  7. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Publication
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  9. pMTL3

    Plasmid
    #127966
    Purpose
    constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127966-plasmid-map-sequence-id-249160

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  10. pMTL5

    Plasmid
    #127967
    Purpose
    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127967-plasmid-map-sequence-id-249184

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  11. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid
    #104571
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    Use
    CRISPR
    Tags
    NLS-3xHA-3xFLAG-4xDmrA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104571-plasmid-map-sequence-id-201436

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  13. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Publication
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  14. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84261
    Purpose
    Expresses AND gate ABA/GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    PYL1-VPR-p2a-GID1-ABI
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84261-plasmid-map-sequence-id-160892

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  17. Ai27

    Plasmid
    #34630
    Purpose
    Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation
    Depositor
    Hongkui Zeng
    Publication
    Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Floxed hChR2(H134R)-tdTomato
    Use
    Mouse Targeting
    Tags
    tdTomato
    Mutation
    His 134 has been mutated to Arg
    Promoter
    CAG
    Available since
    Feb. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    34630-plasmid-map-sequence-id-41505

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  18. pAMW-Wnk

    Plasmid
    #203978
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Tags
    6xMyc
    Expression
    Insect
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203978-plasmid-map-sequence-id-403206

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  19. pAGW-Fray

    Plasmid
    #203979
    Purpose
    GFP-Fray under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Frayed (fray Fly)
    Tags
    EGFP
    Expression
    Insect
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203979-plasmid-map-sequence-id-403220

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  20. pAMW-WnkAAxV

    Plasmid
    #203980
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Tags
    6xMyc
    Expression
    Insect
    Mutation
    RFxV mutated to AAxV
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203980-plasmid-map-sequence-id-403221

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Showing: 1981 - 2000 of 6103 results