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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,729 results for: TIL

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Showing: 3001 - 3030 of 4729 results

  1. pAWPSG-Ptet-nCas9-BE

    Plasmid
    #195739
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Ptet(Tetracycline inducible protein)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195739-plasmid-map-sequence-id-386424

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  8. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid
    #169552
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-NanoLuc Luciferase - mTagBFP fusion
    Expression
    Mammalian
    Promoter
    PPGK
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169552-plasmid-map-sequence-id-364979

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  16. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  19. pCLV3Cas-LT1

    Plasmid
    #210549
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
    Expression
    Plant
    Mutation
    2bp insertion after AAVS1 target sequence that di…
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    210549-plasmid-map-sequence-id-426236

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  20. pCS6-StaPLd-Casp9

    Plasmid
    #111511
    Purpose
    Expresses a tandem caspase-9 dimer, governed by a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    StaPLd-Casp9
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available Since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111511-plasmid-map-sequence-id-214299

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  22. pDL14

    Plasmid
    #106901
    Purpose
    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    Expression
    Bacterial
    Mutation
    Lysine 622 was replaced by Alanine
    Available Since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106901-plasmid-map-sequence-id-334155

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  26. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  27. pFNC-3

    Plasmid
    #233296
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7 and unknown
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233296-plasmid-map-sequence-id-467796

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Showing: 3001 - 3030 of 4729 results