We narrowed to 8,988 results for: Plat
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Plasmid#225141PurposeRecombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 160N, mCherry
TagsAg43 protein fused to silaffin-R5 and mCherryExpressionBacterialPromoterT7Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-AtTIR1-Myc-MBP-AtIAA6-HA
Plasmid#222708PurposeExpression AtTIR1-Myc and MBP-AtIAA6-HA in in bacterial cellsDepositorTagsHA, MBP, and mycExpressionBacterialAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-FCPF
Plasmid#220132PurposeExpresses Cas9-FCPF in E.coliDepositorAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-FCPF
Plasmid#220131PurposeExpresses dCas9-FCPF in mammalian cellsDepositorAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-FCPF
Plasmid#220133PurposeExpresses dCas9-FCPF in E.coliDepositorInsertdCas9-FCPF (cas9 Synthetic, S. pyogenes)
Tags6xHisExpressionBacterialMutationFCPF sequence inserted on C-terminalPromoterT7Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5423_p3WJdB-glcO60
Plasmid#221194PurposeDNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor systemDepositorInsertT7 promoter-glcO60-3WJdB
UseSynthetic BiologyExpressionBacterialPromoterT7 promoter with downstream glcO60 operatorAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1302-AAV-EFSNC-dCjCas9-KRAB
Plasmid#223147PurposeExpression of KRAB with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1312-AAV-EFSNC-dSaCas9-KRAB
Plasmid#223157PurposeExpression of KRAB with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
(R)pGPD1(D)_P1(SBE109)
Plasmid#194993Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)DepositorInsert(R) pGPD (D)
ExpressionBacterialAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a_DIO_HA/FLAG_muMASS
Plasmid#213393PurposeuMASS_1 opioid sensor. AAV Virus production for Cre dependent expressionDepositorInsertuMASS_1
UseAAV and Cre/LoxExpressionMammalianAvailable SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterMutationUTR and partial CDS in-frame with F-LucPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-B'-l2-B'
Plasmid#209119PurposeBacterial expression of CC-GEMS ligand SUMO-B'-l2-B'DepositorInsertSUMO-B'-l2-B'
ExpressionBacterialAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l3-A'
Plasmid#209118PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l3-A'DepositorInsertSUMO-A'-l3-A'
ExpressionBacterialAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l2-G'
Plasmid#209122PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l2-G'DepositorInsertSUMO-A'-l2-G'
ExpressionBacterialAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l2-B'
Plasmid#209121PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l2-B'DepositorInsertSUMO-A'-l2-B'
ExpressionBacterialAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l1-A'
Plasmid#209116PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l1-A'DepositorInsertSUMO-A'-l1-A'
ExpressionBacterialAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-G'-l2-G'
Plasmid#209120PurposeBacterial expression of CC-GEMS ligand SUMO-G'-l2-G'DepositorInsertSUMO-G'-l2-G'
ExpressionBacterialAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l4-G
Plasmid#209123PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-GDepositorInsertSUMO-A'-l4-G
ExpressionBacterialAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l2-A'
Plasmid#209117PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l2-A'DepositorInsertSUMO-A'-l2-A'
ExpressionBacterialAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F)pGPD(G)_P2 (SBE110)
Plasmid#194992Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)DepositorInsert(F) pGPD (G)
ExpressionBacterialAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only