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We narrowed to 15,064 results for: pET

Showing: 5051 - 5100 of 15064 results

  1. pMSP1D1G1

    Plasmid
    #97325
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1G1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of G residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97325-plasmid-map-sequence-id-182623

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  8. pSLS.546

    Plasmid
    #184963
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    priB donor G1069A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184963-plasmid-map-sequence-id-376688

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  9. pSLS.540

    Plasmid
    #184959
    Purpose
    Test effect of a1/a2 length on editing fabH using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fabH donor G954A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184959-plasmid-map-sequence-id-376675

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  12. pMSP1D1T1

    Plasmid
    #97327
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of T residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97327-plasmid-map-sequence-id-182625

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  13. pMSP1D1T2

    Plasmid
    #97328
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T2
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of two T residues after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97328-plasmid-map-sequence-id-182618

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  14. pLz74_Rubisco_CsoS2_Npeptide_fusion

    Plasmid
    #140840
    Purpose
    Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
    Use
    Tags
    CsoS2 N-peptide consensus to CbbL C-terminus and …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140840-plasmid-map-sequence-id-296743

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  15. mGreenLantern-[(A OR S) AND T] OR (C AND V)-SpyTag

    Plasmid
    #243789
    Purpose
    Expresses multifunctional construct enabling the [AND/(OR)]OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9), sortase eSrtA(4S9), TEV, HRV-3C, TEV and TVMV proteases. Construct contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    mGreenLantern
    Use
    Tags
    6x His tag and SsrA
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243789-plasmid-map-sequence-id-486131

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  16. pAM59

    Plasmid
    #227655
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, Δ78–81
    Promoter
    T7
    Available Since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227655-plasmid-map-sequence-id-455288

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  21. pSLS.544

    Plasmid
    #184961
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184961-plasmid-map-sequence-id-376648

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  24. pEUK035

    Plasmid
    #180827
    Purpose
    T7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    SwGdmB
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180827-plasmid-map-sequence-id-355564

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  25. pEUK036

    Plasmid
    #180828
    Purpose
    T7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    CnGdmA
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180828-plasmid-map-sequence-id-355557

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  26. pEUK066

    Plasmid
    #180830
    Purpose
    T7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    NaGdmA
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180830-plasmid-map-sequence-id-355559

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  28. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  39. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Available Since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85090-plasmid-map-sequence-id-164484

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  44. RGD-sfGFP-I27-CC

    Plasmid
    #85392
    Purpose
    MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85392-plasmid-map-sequence-id-172694

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  47. GB1-PRD(703-868, P801G)-Strep

    Plasmid
    #164444
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine
    Promoter
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164444-plasmid-map-sequence-id-324031

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  50. p11_AaC60αβ/C20

    Plasmid
    #229506
    Purpose
    Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Chaperonin 60α
    Chaperonin 60β
    Chaperonin 20
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229506-plasmid-map-sequence-id-456756

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Showing: 5051 - 5100 of 15064 results