We narrowed to 2,545 results for: crispr system

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S9_attL1-EGFP-C-term-attL2

Plasmid

#128473

Purpose

EGFP entry clone

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

EGFP entry clone

Expression

Bacterial

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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S8_attL1-N-term-EGFP-attL2

Plasmid

#128472

Purpose

EGFP entry clones

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

EGFP entry clones

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S1_AscI_V5-gateway_Bsu36I

Plasmid

#128467

Purpose

destination vector with N-terminal V5 tag

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal V5 tag

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S2_AscI_HA-gateway_Bsu36I

Plasmid

#128468

Purpose

destination vector with N-terminal HA tag

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal HA tag

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ179

Plasmid

#85996

Purpose

One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_dCas9-5xGCN4_Hygro

Plasmid

#235572

Purpose

Dox-inducible expression of dCas9-GCN4 & genomic integration

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

dCas9-5xGCN4

Use

CRISPR

Mutation

D10A; H840A

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified bovine U6-2

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBT009.J3.RFP

Plasmid

#177324

Purpose

Activatable RFP expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Insert

mRFP1

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK050

Plasmid

#65157

Purpose

Encodes sgRNA Dropout as a Type 234 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Type

Empty backbone

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

Plasmid

#92099

Purpose

Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Expression

Mammalian

Promoter

Tet-On 3G Bidirectional

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pX330_human CDK1

Plasmid

#118597

Purpose

One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

guide RNA against human CDK1

Use

CRISPR

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-dCas13-APO1-YTH

Plasmid

#204767

Purpose

GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

dCas13-NLS-APO1-YTH-HA

Use

Synthetic Biology

Expression

Mammalian

Promoter

Ef1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCW-tTRE-scFv-SunTag

Plasmid

#193138

Purpose

Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

Depositor

Brad Rosenberg

Article

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

scFv-SunTag

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Csy4-PE2

Plasmid

#172667

Purpose

Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

Csy4-PE2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(AtUBI10)

Plasmid

#202016

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

AtUBI10

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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