We narrowed to 10,167 results for: otos

1. pEB1N-LZ-LOV2(wt)

   Plasmid

   #107614

   Purpose

   N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Use

   Tags

   A. sativa phototropin 1 LOV2 domain and GCN4 leuc…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   CMV

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDF-Az

   Plasmid

   #197101

   Purpose

   To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

   Promoter

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PZac2.1 gfaABC1D-Cx43-GFP

   Plasmid

   #176860

   Purpose

   Expresses Cx43 fused with GFP at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Cx43-eGFP (Gja1 Mouse)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p5E-MCS-c-Fos-b-globin (JDW 1237)

   Plasmid

   #229829

   Purpose

   A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   c-fos minimal promoter and b-globin intron
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PB-GG-OCT4-1-5-PGK-Puro

   Plasmid

   #102893

   Purpose

   PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

   Plasmid

   #173862

   Purpose

   Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)

   Depositor

   Takao Nakata

   Article

   Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

   Insert

   AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-His6-Ubc9-K14R

   Plasmid

   #185759

   Purpose

   Bacterial expression of His6-Ubc9-K14R

   Depositor

   Jeffrey Bode

   Article

   Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   K14R

   Promoter

   T7

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-PPO-Venus

   Plasmid

   #139502

   Purpose

   Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAVS1 KanR foundation lox casette

   Plasmid

   #132388

   Purpose

   Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   G418 resistance
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   promoterless

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiV_Neo_SIK3_CR_T221E

    Plasmid

    #108108

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-FCHO1

    Plasmid

    #196910

    Purpose

    visualization of clathrin-coaed vesicle formation in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

    Insert

    FCHO1 (FCHO1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZac2.1 gfaABC1D-3xHA-SAPAP3

    Plasmid

    #190200

    Purpose

    Expresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    3xHA-SAPAP3 (Dlgap3 Mouse)
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmKO-imp α

    Plasmid

    #200082

    Purpose

    Expresses mKO-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    importin α (KPNA2 Human)
    

    Use

    Tags

    mKO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-CMV-Grid2(I677C)

    Plasmid

    #164782

    Purpose

    cysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Lemoine et al Elife. 2020 Oct 28;9. pii: 59026. doi: 1

    Insert

    Grid2 I677C (Grid2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I677C

    Promoter

    CMV

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe-TetCMV-puro-mVenus-PA-Rac1

    Plasmid

    #22008

    Purpose

    Mammalian expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

    Promoter

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCALNL-mutKir2.1

    Plasmid

    #125579

    Purpose

    Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A non-conducting mutant (GYG -> AAA).

    Promoter

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

    Plasmid

    #176746

    Purpose

    Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    HA-BioID2-BioID2-Kir4.1 (Kcnj10 Aquifex aeolicus, Rat)
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmKikGR Actin

    Plasmid

    #105315

    Purpose

    Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

    Depositor

    Kenneth Yamada

    Article

    Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

    Insert

    actin beta (ACTB Human)
    

    Use

    Tags

    KikGR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21b-LOV-ipaA L514K L531E

    Plasmid

    #40253

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA L514K L531E
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L514K; L531E; Jα helix region hybridized (AsLOV2 …

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only