We narrowed to 4,272 results for: Abo

1. pRSET FLIPglu-600uDelta11 Aphrodite

   Plasmid

   #13569

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A Delta11 Aphrodite
   

   Use

   Tags

   Aphrodite and ECFP

   Expression

   Bacterial

   Mutation

   Fluorophore-derived linker shortened. F16A mutati…

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

   Plasmid

   #117911

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R44A & H45Q on eDHFR

   Promoter

   T7

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRTetR-GAL80

   Plasmid

   #185853

   Purpose

   Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSK226

   Plasmid

   #129268

   Purpose

   dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   Rnq1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR sgSLC19A1

   Plasmid

   #102314

   Purpose

   genetic depletion of SLC19A1

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   sgSLC19A1 (SLC19A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCarCBA

   Plasmid

   #117754

   Purpose

   Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

   Depositor

   Greg Bokinsky

   Article

   Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

   Insert

   Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac(UV5)

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMKO.1-shPrc1

   Plasmid

   #160959

   Purpose

   Prc1 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Prc1 shRNA (Prc1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-P5CR

   Plasmid

   #110295

   Purpose

   Expresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Pyrroline-5-carboxylate Reductase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC027.306

   Plasmid

   #171149

   Purpose

   Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J306 scRNA
   J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J3-BBa_J23117

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CRISPR sgSHMT1_2

    Plasmid

    #106312

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP(1-158) in pcDNAI/Amp

    Plasmid

    #60888

    Purpose

    This vector attaches YFP(1-158) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(1-158)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met was substituted for Gln-69.

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGN33

    Plasmid

    #131105

    Purpose

    An integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutants

    Depositor

    Riccardo Manganelli

    Article

    Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

    Insert

    P-furA102tetO-pip
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    the furA102 promoter was mutated with the inserti…

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSET FLIPglu-600uDelta11 Ares-Aphrodite

    Plasmid

    #13571

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Use

    Tags

    Aphrodite and Ares

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLJC1-Rap2A-3xFLAG

    Plasmid

    #87972

    Purpose

    Expression of Rap2A in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWP1_mFTCD

    Plasmid

    #102317

    Purpose

    expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Ftcd (Ftcd Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-SIRT3

    Plasmid

    #66167

    Purpose

    Expresses SIRT3 via retroviral method

    Depositor

    Ronald Kahn

    Article

    Jing et al Diabetes. 2013 Oct;62(10):3404-17. doi:

    Insert

    SIRT3 (Sirt3 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    L235P

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pSECB

    Plasmid

    #106305

    Purpose

    Express spCas9 with sgRNA, blast resistance

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6_Ptrc_BsMdh_BmHps_BmPhi

    Plasmid

    #129104

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLenti CRISPR V2 sgShmt2_2

    Plasmid

    #106315

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting mShmt2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

    Plasmid

    #117909

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R98A on eDHFR

    Promoter

    T7

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge