We narrowed to 11,729 results for: pcDNA

1. pcDNA3-Lyn-dInPAkt

   Plasmid

   #181834

   Purpose

   Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-dInPAkt
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-StrepHA-SUMO2

   Plasmid

   #66867

   Purpose

   Mammalian expression of SUMO2 with a strep and an HA tag

   Depositor

   Ying Liu

   Article

   Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA and Strep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. K44A HA-dynamin 1 pcDNA3.1

   Plasmid

   #34683

   Depositor

   Sandra Schmid

   Insert

   dynamin 1ba (DNM1L Human)
   

   Use

   Tags

   HA (variant)

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1.Gαq-LgB97

   Plasmid

   #134360

   Purpose

   Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαq-LgB97 (GNAQ Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. c-Rel cFlag pcDNA3

   Plasmid

   #20013

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Insert

   c-Rel (Rel Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-HA-mSTING (N153S)

   Plasmid

   #214158

   Purpose

   Transient expression of gain-of-function STING mutant

   Depositor

   Nan Yan

   Article

   Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

   Insert

   STING (N153S) (Sting1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N153S

   Promoter

   CMV

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.4_1G11F2-3 light chain

   Plasmid

   #198254

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3962 pcDNA3-IRF3-FL

   Plasmid

   #22860

   Depositor

   Peter Howley

   Article

   Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

   Insert

   IRF3 (IRF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-human OCRL

   Plasmid

   #22207

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Oculocerebrorenal syndrome of Lowe (OCRL Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 TAP DHX36

    Plasmid

    #53547

    Purpose

    mammalian expression of DHX36

    Depositor

    Kathleen Collins

    Article

    Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO V5 HSPA1L

    Plasmid

    #19511

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 1-like (HSPA1L Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-AUP1-FLAG 5E

    Plasmid

    #185341

    Purpose

    Assessing G2BR function in AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    Flag, 6xHis

    Expression

    Mammalian

    Mutation

    R382E/Q383E/K390E/R398E/R400E

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Bsd-mEGFP

    Plasmid

    #214369

    Purpose

    Expression of the monomeric Green Fluorescent Protein

    Depositor

    Bertrand Collet

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-myc3-bTrCP

    Plasmid

    #20718

    Depositor

    Yue Xiong

    Article

    Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

    Insert

    beta-transducin repeat containing (BTRC) (Btrc Mouse)
    

    Use

    Tags

    Myc3

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-ALK-V5/HIS

    Plasmid

    #200115

    Purpose

    Expression of human ALK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ALK receptor tyrosine kinase (ALK Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

    Plasmid

    #159127

    Purpose

    expresses FBXO22 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    FBXO22 (FBXO22 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-myc3-p27

    Plasmid

    #19937

    Depositor

    Yue Xiong

    Article

    Zariwala et al Oncogene. 1998 Nov 26. 17(21):2787-98.

    Insert

    CDKN1B cyclin-dependent kinase inhibitor 1B (CDKN1B Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-mCherry-Rheb(5A)

    Plasmid

    #192439

    Purpose

    Encodes mCherry-tagged Rheb lacking residues 38-42.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Rheb amino acids 38-42 deleted.

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Myc-AIM2

    Plasmid

    #73958

    Purpose

    Express c-Myc-tagged human AIM2 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

    Insert

    absent in melanoma 2 (AIM2) (AIM2 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-GFP11(x7)-Actin

    Plasmid

    #181967

    Purpose

    Human beta actin tagged with 7 copies of GFP11.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    GFP11(x7)-Actin (ACTB )
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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