We narrowed to 4,415 results for: ado

1. pLenti-PGK-RAP2A-N17-neo

   Plasmid

   #156179

   Purpose

   Lentiviral vector for expression of RAP2A-N17, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23 C3_9

   Plasmid

   #60295

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   NKX6-1 enhancer (NKX6-1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLentiCMVblast_SLC38A2_N82A

   Plasmid

   #156181

   Purpose

   CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   CMV

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pInducer20_SLC38A2_N82A

   Plasmid

   #156183

   Purpose

   Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   TRE

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

   Plasmid

   #156405

   Purpose

   Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4A-WT (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   mScarlet-I

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.23 C3_29

   Plasmid

   #60309

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   ZFP36L2 enhancer (ZFP36L2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pENTR D-TOPO-C3_10

   Plasmid

   #60259

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   RFX6 enhancer (RFX6 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR D-TOPO-C3_25

   Plasmid

   #60268

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   ROCK1 enhancer (ROCK1 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ai14-luc

   Plasmid

   #87118

   Purpose

   AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
   

   Use

   AAV, CRISPR, and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL4.23 C3_31

    Plasmid

    #60310

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    SLC2A2 enhancer (SLC2A2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pGL4.23 C3_21

    Plasmid

    #60296

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    CDKN2BAS enhancer (CDKN2B-AS1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pGL4.23 C3_19

    Plasmid

    #60302

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    THADA enhancer (THADA Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET28b-Hf-RfxCas13d-His

    Plasmid

    #234480

    Purpose

    Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed four alanine to valine in positions 134, …

    Promoter

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-multi-CRISPR-sgRipk1_#1-puro

    Plasmid

    #231984

    Purpose

    Knockout mouse Ripk1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-multi-CRISPR-sgRipk1_#2-puro

    Plasmid

    #231983

    Purpose

    Knockout mouse Ripk1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-HNF1B-FUS-IDR

    Plasmid

    #183240

    Purpose

    Expression of HNF1B fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox B (HNF1B Human)
    

    Use

    Tags

    Fusion to an IDR from the FUS protein and FLAG ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-HNF1A-delta-IDR2

    Plasmid

    #183238

    Purpose

    Expression of FLAG tagged HNF1A with deletion of a intrinsically disordered regions (AA545-573)

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox A (HNF1A Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 545-573

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP-nE-mHP1alpha

    Plasmid

    #181895

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGFP-nE-mHP1alpha-delCTE

    Plasmid

    #181896

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha-delCTE (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFP-FUSN-mHP1alpha

    Plasmid

    #181897

    Purpose

    Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    FUSN-mHP1alpha (Cbx5 Human, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge