We narrowed to 2,475 results for: T2A

1. pcDNA3.1 Mfn2-HA S27D

   Plasmid

   #139194

   Purpose

   Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

   Depositor

   Allan Weissman

   Article

   Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

   Insert

   MFN2 (MFN2 Human)
   

   Tags

   2HA

   Expression

   Mammalian

   Mutation

   Serine 27 to Aspartic Acid

   Promoter

   CMV promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

   Plasmid

   #227608

   Purpose

   Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   aa 2-83 deleted

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPICZ A-SLC-His+MFN2-Strep (SB255)

   Plasmid

   #227604

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

   Plasmid

   #227609

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-544, 651-757 only [TM deleted]

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

   Plasmid

   #227611

   Purpose

   Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   MBP, 10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-88 only

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

   Plasmid

   #227610

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-400, 706-757 [TM and HB2 deleted]

   Promoter

   AOX1

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ10878

   Plasmid

   #183964

   Purpose

   CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D156R, E292R, D235R, D350R

   Promoter

   CAG

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-TetO-hNGN2-eGFP-Puro

   Plasmid

   #79823

   Purpose

   Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

   Depositor

   Kristen Brennand

   Article

   Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

   Insert

   hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
   

   Use

   Lentiviral

   Promoter

   TetO

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PEmax_AAVS1_knockin_HDR_donor

   Plasmid

   #223000

   Purpose

   HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PEmax
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ269E2

    Plasmid

    #164721

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225697

    Purpose

    Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (WT) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Plenti-ARE-HSVTK-luc

    Plasmid

    #161789

    Purpose

    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

    Depositor

    Hans-Guido Wendel

    Article

    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

    Inserts

    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EF1a and Murine Gclc ARE

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. IronFistMUT.

    Plasmid

    #243008

    Purpose

    Iron binding deficient IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    hemerythrin-like-mNeonGreen (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    H15A, H57A, E58A, E61A, H126A, E130A

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1a-eFFly-eGFP

    Plasmid

    #104834

    Purpose

    eFFly luciferase for invivo Imaging, eGFP for flourescent detection

    Depositor

    Irmela Jeremias

    Article

    Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

    Inserts

    eFFly
    eGFP
    

    Use

    Lentiviral and Luciferase; Egfp

    Expression

    Mammalian

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBA904

    Plasmid

    #122238

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

    Plasmid

    #229545

    Purpose

    Lentiviral construct to express rtTA and SKM proteins

    Depositor

    Domitilla Del Vecchio

    Article

    Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

    Insert

    rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EWSR1::WT1(-KTS)

    Plasmid

    #220565

    Purpose

    Lentiviral expression of human EWSR1::WT1(-KTS)

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    EWSR1::WT1(-KTS) (EWSR1, WT1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

    Plasmid

    #111818

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Mef2c (Mef2c Mouse)
    Tdtomato
    Gata4 (Gata4 Mouse)
    Tbx5
    

    Use

    Cloning intermediate

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBA900

    Plasmid

    #122237

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs2 at the 3' end of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-EWSR1::WT1(+KTS)

    Plasmid

    #220566

    Purpose

    Lentiviral expression of human EWSR1::WT1(+KTS)

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    EWSR1::WT1(+KTS) (WT1, EWSR1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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