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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,025 results for: plasmid dna

Showing: 2061 - 2080 of 6025 results

  1. ABE7.10-F148A

    Plasmid
    #132946
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    ABE7.10(F148A)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    ABE7.10(F148A)
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    132946-plasmid-map-sequence-id-314827

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  2. AAV-short_BrokenHeart (no BC)

    Plasmid
    #86950
    Purpose
    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.
    Depositor
    Joseph Dougherty, Rob Mitra
    Article
    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    Brokenheart construct
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    86950-plasmid-map-sequence-id-172437

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  3. BE3-hA3A-R128A

    Plasmid
    #132944
    Purpose
    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-hA3A(R128A)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    BE3-hA3A(R128A)
    Promoter
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132944-plasmid-map-sequence-id-314824

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  4. BE3-hA3A-Y130F

    Plasmid
    #132945
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-hA3A(Y130F)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    BE3-hA3A(Y130F)
    Promoter
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132945-plasmid-map-sequence-id-314826

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  5. pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

    Plasmid
    #215026
    Purpose
    Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    Depositor
    Joanna Wysocka
    Article
    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4X E-box mutations within Coordinator motifs
    Promoter
    SV40
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    215026-plasmid-map-sequence-id-425844

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  7. BE3-YE1

    Plasmid
    #132943
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-(W90Y-R126E)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    BE3-(W90Y-R126E)
    Promoter
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132943-plasmid-map-sequence-id-314816

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  8. JARID1BA

    Plasmid
    #74646
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JARID1BA (KDM5B Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    Leu101-(Gly)4-Ala374 deletion mutant
    Promoter
    PH
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. JARID1BA

    Plasmid
    #74648
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JARID1BA (KDM5B Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    Leu101-(Gly)4-Ala374 deletion mutant
    Promoter
    PH
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pNIC28-hKdm2b-CxxC-PHD

    Plasmid
    #49216
    Purpose
    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.
    Depositor
    Rob Klose
    Insert
    Kdm2b (KDM2B Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49216-plasmid-map-sequence-id-72771

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  18. DDI1

    Plasmid
    #32867
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DDI1 (DDI1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    amino acids 239-367 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

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Showing: 2061 - 2080 of 6025 results