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NES-mCherry-P4Mx1

Plasmid

#108143

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Vamp2-pHluorin

Plasmid

#190151

Purpose

Expresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosis

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ ∂CC

Plasmid

#24816

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 222-265 (coiled coil doma…

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pICH47772

Plasmid

#48004

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAG79-attL1-pegRNA-attL2

Plasmid

#213050

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-bNWASP

Plasmid

#33019

Depositor

Marc Kirschner

Article

Lee et al Science. 2010 Sep 10;329(5997):1341-5.

Insert

N-WASP (WASL Bovine)

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pT2HLacZpA1I.7 (#59)

Plasmid

#35965

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter (Tek Mouse)
SDK LacZ
SV40 pA
Tie2 full enhancer

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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TOPO Cre-2A-GFP

Plasmid

#68450

Purpose

TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cre-2A-GFP

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Dcp1b

Plasmid

#153976

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1b

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA recapping enzybe 1b (DCP1B Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-p14FAST

Plasmid

#50025

Purpose

pENTR vector for reptilian orthoreovirus p14 fusion-associated small transmembrane (FAST) protein

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

p14FAST

Use

Gateway Cloning

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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TAL2038

Plasmid

#39396

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pEGFPN1-RBM3

Plasmid

#87860

Purpose

Expresses human RBM3 coupled to GFP

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

RNA binding motif protein 3 (RBM3 Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMU-PERr

Plasmid

#61202

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBS448 RSV-GFPcre

Plasmid

#11919

Purpose

Expresses Cre-GFP fusion under the control of the Rous sarcoma virus (RSV) LTR/promoter

Depositor

Brian Sauer

Article

Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
Kv7.1/pcDNA3.1

Plasmid

#111452

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)

Tags

8xHis and EGFP

Expression

Mammalian

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a-QL

Plasmid

#86076

Purpose

A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamine 67 to leucine

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-T31N (DN)

Plasmid

#83423

Purpose

Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residue 31 from T to N (T31N: ACG -> AAC)

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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726-Desmoglein2-myc

Plasmid

#32232

Depositor

Kathleen Green

Article

Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

Insert

Desmoglein2 (DSG2 Human)

Tags

Myc

Expression

Mammalian

Promoter

human beta-actin

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pSicoR-mCh-Oct4i

Plasmid

#21906

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Oct4i

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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