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pGEX-4T3-ZO-2-1st-PDZ-delta-NLS
Plasmid#27425DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationDeletion of NLS-Nuclear Localization Signal: Nucl…PromoterAvailable sinceMay 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 LNG (CC→SS)-6MT
Plasmid#41739DepositorInsertNotch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationContains murine Notch-1 leader peptide (aa 1-23) …PromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS CMV NA 3C FLAG SA
Plasmid#234993PurposeExpression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA
UseTagsHRV 3C site, FLAG, Strep-Tactin (SA)ExpressionMammalianMutationIn the Streptavidin coding sequence Glu44Val/Ser4…PromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvHA
Plasmid#234991PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinsDepositorInsertNA-3C-myc-scfvHA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Pchi-Q61A
Plasmid#231520PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generationDepositorInsertPchi HMG Q61A flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutation at position 61 Q to A of the HMG d…PromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Pchi-Q61A/E64M
Plasmid#231521PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generationDepositorInsertPchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutations at position 61 Q to A and 64 E to…PromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMBS-845
Plasmid#218000PurposeStrep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsertStrep-tag II
UseSynthetic BiologyTagsPreScission protease cleavage siteExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-846
Plasmid#218001Purpose3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsert3xFLAG-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-847
Plasmid#218002PurposeMyc-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsertMyc-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
Plasmid#218916PurposeLentiviral construct expressing msfGFP and nuclear mCherry proteins.DepositorInsertEF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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KIF13B 1-584
Plasmid#163908PurposeMotile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.DepositorInsertkif13b (Kif13b Mouse)
UseTagsCleavable 6 x HIS tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 MUT
Plasmid#110126PurposeExpresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activityDepositorInsertURH49 (DDX39A Human)
UseTagsFlagExpressionInsectMutation4 amino acid mutations (RSFS motif changed to KSL…PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E MUT
Plasmid#110122PurposeExpresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutation4 mutations (KKLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
NKP91 GFP (1034)
Plasmid#62023Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTmem41A (TMEM41A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP13
Plasmid#159614PurposeBacterial expression plasmid for COVID-SARS2 NSP13 helicaseDepositorInsertCovid-SARS2 Nsp13
UseTagsHis6-Zb-TEVExpressionBacterialMutationCodon-optimized for E. coli expressionPromoterT7 - LacOAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NLS-actin
Plasmid#118380PurposeGateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationNLSPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)
Plasmid#182395PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusionsDepositorInsertsVNp-LZ-VenusN154
VNp-LZ-VenusC155
UseTagsVesicle Nucleating peptide (VNp) & Leucine Zi…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only
