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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 2081 - 2100 of 3270 results

  1. Sniper-Cas9

    Plasmid
    #138559
    Purpose
    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    Sniper-Cas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138559-plasmid-map-sequence-id-298901

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  2. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

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  3. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  4. YE1-BE4max-CP1028

    Plasmid
    #138160
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138160-plasmid-map-sequence-id-272345

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  7. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  9. pRS0045

    Plasmid
    #131124
    Purpose
    Mammalian Target-ACE expression vector
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1, TadA, and UGI
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131124-plasmid-map-sequence-id-257454

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  10. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

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  11. A3A-PBE-ΔUGI

    Plasmid
    #119770
    Purpose
    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)
    Depositor
    Caixia Gao
    Publication
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-ΔUGI-NLS
    Tags
    NLS
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119770-plasmid-map-sequence-id-232089

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  12. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

    Plasmid
    #113436
    Purpose
    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    type II-C anti-CRISPR
    Use
    CRISPR
    Tags
    FLAG/NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113436-plasmid-map-sequence-id-225078

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  13. pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107318
    Purpose
    Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107318-plasmid-map-sequence-id-204674

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  14. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid
    #107320
    Purpose
    Expresses SpCas9 fused to SaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to SaCas9
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107320-plasmid-map-sequence-id-204686

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  18. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

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  20. PP7 guide

    Plasmid
    #86168
    Purpose
    Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells
    Depositor
    Amit Choudhary
    Publication
    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7 guide
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86168-plasmid-map-sequence-id-171565

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Showing: 2081 - 2100 of 3270 results