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C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pQE30-His-IF3

Plasmid

#124125

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 3 (infC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GlnRS-His

Plasmid

#124108

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutaminyl-tRNA ligase (glnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG.PKCzeta.K/W

Plasmid

#10800

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K281W (Lys 281 to Trp)

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pINDUCER-21-RUNX1

Plasmid

#97043

Purpose

Expresses RUNX1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pCDNA3-STAT3-S727D

Plasmid

#73364

Purpose

Expression of STAT3-S727D

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Tags

FLAG tagged

Expression

Mammalian

Mutation

E16K S727D

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-Ecadherin

Plasmid

#65287

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Foxp1A

Plasmid

#16362

Depositor

Anjana Rao

Article

Hu et al Nat Immunol. 2006 Aug . 7(8):819-26.

Insert

FOXP1A (Foxp1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-BFP-24xPP7

Plasmid

#74929

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-BFP-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Chronos-GFP

Plasmid

#58805

Purpose

AAV expression of Chronos-GFP under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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pQP-T2A

Plasmid

#102583

Purpose

Light-controlled activation of expression

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

Insert

BphP1-VP16-T2A-NLS-GAL4-QPAS1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-mCherry

Plasmid

#29585

Depositor

Anna Huttenlocher

Article

Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

Insert

mpx-mCherry

Use

Zebrafish expression

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pCM1.127

Plasmid

#21384

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pie-1 promoter (short, no ATG) in pDONRP4P1R

Use

Gateway Cloning

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc card9

Plasmid

#16253

Depositor

William Kaelin

Article

Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

Insert

card9 (CARD9 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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1.5 BV

Plasmid

#16048

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter region (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1585 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
GLUT1-GFP

Plasmid

#89440

Purpose

GLUT1 construct

Depositor

Jeffrey Rathmell

Article

Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

Insert

GLUT1 (Slc2a1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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