We narrowed to 5,312 results for: pcDNA3.1(+)

1. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

   Plasmid

   #163990

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1461R

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

   Plasmid

   #72888

   Purpose

   fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

   Insert

   Gaussia luciferase, Channelrhodopsin-2, EYFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

   Plasmid

   #187089

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-SF-iGluSnFR
   

   Use

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127315

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

   Plasmid

   #53551

   Purpose

   mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   contains aa 88-544, with aa 166-172 replaced wit…

   Promoter

   CMV

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

   Plasmid

   #187091

   Purpose

   GABA sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-iGABASnFR
   

   Use

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-iRFP-P2A-Post-SPHERE-iGABASnFR

   Plasmid

   #187092

   Purpose

   GABA sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-iGABASnFR
   

   Use

   Tags

   iRFP670-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)

   Plasmid

   #169126

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hNrf1 (NFE2L1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NAWLVH106-AAAAAA

   Promoter

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR

   Plasmid

   #187090

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-SF-iGluSnFR
   

   Use

   Tags

   iRFP670-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

    Plasmid

    #70668

    Purpose

    Human mutant of E2F1 lacking amino acids 2 to 126

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 2 to 126 (E2F1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    lacks amino acids 2-126

    Promoter

    cmv

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194750

    Purpose

    To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid

    #160742

    Purpose

    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

    Plasmid

    #181814

    Purpose

    Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc5b D1 (Muc5b Mouse)
    

    Use

    Tags

    TEV cleavage site followed by 6xHistidine tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. (282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

    Plasmid

    #160741

    Purpose

    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nGFP-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A

    Plasmid

    #115843

    Purpose

    Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251-Vpx-Q76A
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Q76A

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

    Plasmid

    #166798

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    LL100EE

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

    Plasmid

    #158604

    Purpose

    Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

    Plasmid

    #187994

    Purpose

    Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    mucin2 D1D2D'D3 (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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