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pGEX4T-1_OCT4_T234A_S235A

Plasmid

#40634

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Mammalian

Mutation

T234A_S235A

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234E_S235E

Plasmid

#40635

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

T234E_S235E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234E

Plasmid

#40636

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

T234E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234A

Plasmid

#40637

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Mutation

T234A

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_S235E

Plasmid

#40638

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

S235E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_S235A

Plasmid

#40639

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…

Expression

Bacterial

Mutation

S235A

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
3187 pSFFV-neo Bad cDNA ORF

Plasmid

#8756

Depositor

Stanley Korsmeyer

Article

Zha et al Cell 1996 Nov 15;87(4):619-28.

Insert

BAD (Bad Mouse)

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pFIN-IRBP156-tdTOM

Plasmid

#44356

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

IRBP156-tdTOM

Use

Lentiviral

Promoter

murine interphotoreceptor retinoid-binding protei…

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 BimL (S58A)

Plasmid

#24249

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(S58A) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pSBbi-MTS-FAP-GFP

Plasmid

#242990

Purpose

Expresses MTS-FAP-GFP in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

MTS-dH6.2-GFP

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi-FAP-GFP-KDEL

Plasmid

#242991

Purpose

Expresses FAP-GFP-KDEL in mammalian cells

Depositor

Itay Budin

Article

Moore et al Nat Chem Biol (2025). https://doi.org/10

Insert

dH6.2-GFP-KDEL

Use

Sleeping beauty

Tags

GFP

Expression

Mammalian

Promoter

EF-1⍺

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHYG

Plasmid

#243678

Purpose

Expresses the HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator

Depositor

Patrick Krysan

Article

De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.

Insert

HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS

Expression

Plant

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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XAC1 S3A,Y82F

Plasmid

#245949

Purpose

Expressing constitutively active XAC1 with lower turnover rate

Depositor

James Bamburg

Article

Abe et al J Cell Biol. 1996 Mar;132(5):871-85. doi

Insert

cofilin 1 (cfl1) (cfl1.L Frog)

Use

Frog expression

Mutation

S3A, Y82F

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mStayGold

Plasmid

#234549

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mStayGold(Ando) fusion protein

Depositor

Hajime Hirase

Article

Knak et al Neurophotonics, Vol. 12, Issue S2, S2281

Insert

Alb-mStayGold

Use

AAV

Mutation

None

Promoter

P3

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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huADF S3A

Plasmid

#245943

Purpose

Expressing a constitutively active human ADF

Depositor

James Bamburg

Article

Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7

Insert

actin depolymerizing factor (ADF) (DSTN Human)

Expression

Mammalian

Mutation

S3A

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) S44A

Plasmid

#206029

Purpose

Expresses the SNARE motif of human VAMP3 mutant S44A in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Mutation

changes serine 44 to alanine

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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hVAMP3(1-77) S44E

Plasmid

#206030

Purpose

Expresses the SNARE motif of human VAMP3 mutant S44E in bacteria

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Mutation

changes serine 44 to glutamic acid

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-GalK-USP-ET64

Plasmid

#234662

Purpose

Expression an ELP-tagged fusion enzyme of GalK and AtUSP

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

galactokinase
UDP-sugar pyrophosphorylase (USP Mustard Weed)

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMH-SpAsp1

Plasmid

#238315

Purpose

SpAsp1 protein expression in bacteria

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

asp1 (asp1 Fission Yeast)

Tags

His7-Tag, Strep-tag II, SUMO protein

Expression

Bacterial

Mutation

SpAsp1

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBN201

Plasmid

#217917

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations

Depositor

James Naismith

Article

Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y715E, Y717E, Y718E

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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