We narrowed to 15,064 results for: pET
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Plasmid#222212PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tagDepositorInsertfghA(W15C)
UseTags6x HisExpressionBacterialMutationChanged Tryptophan 15 to CysteinePromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FENEF-Sensor
Plasmid#208930Purposein vitro p38-Sensor for luminescent detection of p38-activityDepositorInsertFENEF sequence from human ATF2 with phospho site (ATF2 Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-CEP55(160-217, S215C)
Plasmid#186793PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CEP55(160-217, S215C)DepositorInsertGB1-His6-TEV-CEP55(160-217, S215C) (CEP55 Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS215CPromoterAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)
Plasmid#199242PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS191C, I225A, L228A, W231APromoterAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4B(S184C)
Plasmid#180026PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).DepositorInsertGB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS184CPromoterAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
DNMT2Δ191-237
Plasmid#198382PurposeBacterial expression plasmid for human DNMT2 deletion mutant; for binding assaysDepositorInsertDNMT2 (TRDMT1 Human)
UseTagsHis-tagExpressionBacterialMutationdeleted amino acids 191-237PromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppil2.280.457.Y389H
Plasmid#137658PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppil2 (PPIL2 Human)
UseTags(His)6-thrombinExpressionBacterialMutation280-457-Y389HPromoterAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppil2.280.457.Y389W
Plasmid#137657PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppil2 (PPIL2 Human)
UseTags(His)6-thrombinExpressionBacterialMutation280-457-Y389WPromoterAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIS_TEV_hsSYX3
Plasmid#179290PurposeE. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7lacAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, T805C, C813S)-Strep
Plasmid#169749PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Threonine 805 to Cysteine…PromoterAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, G808C, C813S)-Strep
Plasmid#169750PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Glycine 808 to Cysteine, …PromoterAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, S712C, P801G, C813S)-Strep
Plasmid#164445PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationSerine 712 to Cysteine, Proline 801 to Glycine, C…PromoterAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, A756C, P801G, C813S)-Strep
Plasmid#164446PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationAlanine 756 to Cysteine, Proline 801 to Glycine, …PromoterAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, C813S, S863C)-Strep
Plasmid#164447PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Cysteine 813 to Serine, S…PromoterAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10, RGE)-I27-CC
Plasmid#85396PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationGRGDS motif mutated to GRGESPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
KS6A1_HUMAN_D1
Plasmid#79720PurposeThis plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1G3_HUMAN_D0
Plasmid#79685PurposeThis plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1187
Plasmid#119256Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1189
Plasmid#119257Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1346
Plasmid#119274Purposeknockdown folA in K. pneumoniaeDepositorInsertsgRNA folA (K. pneumoniae)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1159
Plasmid#119250Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1356
Plasmid#119276Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1237
Plasmid#119262Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1183
Plasmid#119254Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1185
Plasmid#119255Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1358
Plasmid#119277Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1354
Plasmid#119275Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1333
Plasmid#119268Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1360
Plasmid#119278Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1223
Plasmid#119261Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1221
Plasmid#119260Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1335
Plasmid#119269Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1104
Plasmid#119248Purposeknockdown pqsC in P. aeruginosaDepositorInsertsgRNA pqsC (P. aeruginosa)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1363
Plasmid#119279Purposefor stabilizing ICE in the presence of rapI expressionDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))
Plasmid#164262PurposeVector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.DepositorInsertCodon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1171
Plasmid#119253Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1170
Plasmid#119252Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1217
Plasmid#119258Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1069
Plasmid#119244Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1161
Plasmid#119251Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTP130 (pTA106_TnsABC(Tn6900))
Plasmid#164261PurposeVector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.DepositorInserttnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutation(tnsA) Changed Alanine 125 to Aspartic acidPromoterLactoseAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1071
Plasmid#119245Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1290
Plasmid#119267Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1067
Plasmid#119243Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1102
Plasmid#119246Purposeknockdown phzA1 in P. aeruginosaDepositorInsertsgRNA phzA1 (P. aeruginosa)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1103
Plasmid#119247Purposeknockdown phzM in P. aeruginosaDepositorInsertsgRNA phzM (P. aeruginosa)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1055
Plasmid#119242Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationPromoterAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only