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We narrowed to 15,064 results for: pET

Showing: 5251 - 5300 of 15064 results
  1. pACB113

    Plasmid
    #222212
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag
    Depositor
    Insert
    fghA(W15C)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Tryptophan 15 to Cysteine
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)

    Plasmid
    #199242
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)
    Depositor
    Insert
    GB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
    Use
    Tags
    GB1-His6-TEV
    Expression
    Bacterial
    Mutation
    S191C, I225A, L228A, W231A
    Promoter
    Available Since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. HIS_TEV_hsSYX3

    Plasmid
    #179290
    Purpose
    E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site
    Depositor
    Insert
    Syx
    Use
    Tags
    His6 + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7lac
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. GB1-PRD(703-868, P801G, T805C, C813S)-Strep

    Plasmid
    #169749
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Threonine 805 to Cysteine…
    Promoter
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. GB1-PRD(703-868, P801G, G808C, C813S)-Strep

    Plasmid
    #169750
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Glycine 808 to Cysteine, …
    Promoter
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. GB1-PRD(703-868, S712C, P801G, C813S)-Strep

    Plasmid
    #164445
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Serine 712 to Cysteine, Proline 801 to Glycine, C…
    Promoter
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. GB1-PRD(703-868, A756C, P801G, C813S)-Strep

    Plasmid
    #164446
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Alanine 756 to Cysteine, Proline 801 to Glycine, …
    Promoter
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. GB1-PRD(703-868, P801G, C813S, S863C)-Strep

    Plasmid
    #164447
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.
    Depositor
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Cysteine 813 to Serine, S…
    Promoter
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. FN(9-10, RGE)-I27-CC

    Plasmid
    #85396
    Purpose
    MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Insert
    Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    GRGDS motif mutated to GRGES
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
  13. KS6A1_HUMAN_D1

    Plasmid
    #79720
    Purpose
    This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Insert
    KS6A1 (RPS6KA1 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  14. KC1G3_HUMAN_D0

    Plasmid
    #79685
    Purpose
    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Insert
    KC1G3 (CSNK1G3 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  15. pMTP130 (pTA106_TnsABC(Tn6900))

    Plasmid
    #164261
    Purpose
    Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.
    Depositor
    Insert
    tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    (tnsA) Changed Alanine 125 to Aspartic acid
    Promoter
    Lactose
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 5251 - 5300 of 15064 results