We narrowed to 5,339 results for: 290

1. pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

   Plasmid

   #164184

   Purpose

   Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

   Depositor

   Thomas Oertner

   Article

   Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

   Inserts

   CamPARI2
   Human α Tubulin
   

   Use

   AAV

   Tags

   6 His tag and NES

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mouse CENP-B-EGFP

   Plasmid

   #107264

   Purpose

   mouse CENP-B-EGFP in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   CENP-B-EGFP (Cenpb Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBtPIPLC His

   Plasmid

   #173808

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Mary F Roberts, Anne Gershenson

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. hHSF1 K80Q

   Plasmid

   #118351

   Purpose

   Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

   Depositor

   Lea Sistonen

   Article

   Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K80Q

   Promoter

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIP EGFP-ER [TM(SAC1)]

   Plasmid

   #186475

   Purpose

   Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   SAC1 (SACM1L Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-terminal transmembrane region of the protein: 5…

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pInducer20 CARD8-HA PAM

   Plasmid

   #169982

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

   Plasmid

   #164013

   Purpose

   Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R464E

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-FKBP-SAC1-HLx2

   Plasmid

   #108133

   Purpose

   Recruitable SAC1 with extended linker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SACM1L(1-520):[EAAAR]2:SACM1L(521-587)

   Promoter

   CMV

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. H52-pUCM-hNGN2-inv

   Plasmid

   #110492

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

   Depositor

   Michael Ward

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mcherry
   rtTA3G
   

   Use

   CRISPR, Cre/Lox, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, EF-1 alpha, and TRE3G

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MYC-hTRAK1

    Plasmid

    #225204

    Purpose

    Expresses MYC-tagged human TRAK1

    Depositor

    Thomas Schwarz

    Article

    Pekkurnaz et al Cell. 2014 Jul 3;158(1):54-68. doi: 10.1

    Insert

    trak1 (TRAK1 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid

    #105200

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIG PIK3CA E545K

    Plasmid

    #73055

    Purpose

    Human PIK3CA E545K

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E545K

    Promoter

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX305-N-dTAG-CARD8

    Plasmid

    #169989

    Purpose

    Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    dTAG

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid

    #128746

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 point mutations disrupting the miR-7 site

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

    Plasmid

    #66789

    Purpose

    Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRalpha (Pdgfra Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D842V (constitutively active)

    Promoter

    EF1a

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

16. Teto Ngn2

    Plasmid

    #62223

    Purpose

    Inducible expression of Ngn2

    Depositor

    Kristin Baldwin

    Article

    Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

    Insert

    Neurog2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(-)-LZ-CD95L

    Plasmid

    #104349

    Purpose

    Expresses human CD95L leucine zipper in mammalian cells

    Depositor

    Alexander McLellan

    Article

    Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

    Insert

    CD95L leucine zipper (FASLG Human)
    

    Use

    Tags

    octahistidine tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-Ahi1

    Plasmid

    #30494

    Depositor

    Joseph Gleeson

    Article

    Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

    Insert

    Ahi1 (AHI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX6P1-UBE2S-UBD

    Plasmid

    #66713

    Purpose

    Expresses the UBE2S-UBD fusion protein in E. coli

    Depositor

    David Komander

    Article

    Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

    Inserts

    e2-epf (UBE2S )
    isot (USP5 )
    

    Use

    Tags

    GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCIG PIK3CA Wildtype

    Plasmid

    #73056

    Purpose

    Human PIK3CA Wildtype

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    PIK3CA Wildtype (PIK3CA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge