166,818 results

1. pLNHA-C1-Hs4E-BP1

   Plasmid

   #79438

   Purpose

   Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Hs4E-BP1 (EIF4EBP1 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT-FLAG-HA-(N)-CDK12

   Plasmid

   #231750

   Purpose

   Expression in Flp-IN human cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   CDK12 (CDK12 Human)
   

   Use

   Tags

   Flag-HA-3C

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. ins:CFP-NTR

   Plasmid

   #66593

   Purpose

   Specific and conditional cell ablation of beta cells in the zebrafish pancreas

   Depositor

   Didier Stainier

   Article

   Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

   Insert

   Insulin promotor driving CFP-nitroreductase
   

   Use

   Zebrafish expression

   Tags

   CFP fused with N-terminus of nitroreductase

   Expression

   Mutation

   Promoter

   Zebrafish Insulin promoter (approximately 1.2kb)

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP-hCB2R

   Plasmid

   #179322

   Purpose

   Express human cannabinoid 2 receptor (GFP tagged) in mammalian cells

   Depositor

   Christian Gruber

   Article

   Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

   Insert

   human cannabinoid 2 receptor (CNR2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP3545-dCas9Str

   Plasmid

   #153516

   Purpose

   Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   dCas9Str
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   nisA

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVC-Ds-MCS-Ds

   Plasmid

   #102416

   Purpose

   Empty vector with a multiple cloning site flanked by Ds integration elements

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-REPORT(EF1a)

   Plasmid

   #172326

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11d-RAN

   Plasmid

   #108919

   Purpose

   Expresses human RAN

   Depositor

   Larry Gerace

   Article

   Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

   Insert

   RAN (RAN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP1-bio

   Plasmid

   #47709

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP1 (MSP1 Synthetic, Plasmodium falciparum)
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (AAV9)

    Viral Prep

    #112007-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (#112007). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6s and bicistronic mRuby3. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Promoter

    Syn

    Tags

    mRuby3

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

11. EC71174

    Plasmid

    #154067

    Purpose

    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Inserts

    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    

    Use

    Cre/Lox and Synthetic Biology; Golden gate

    Tags

    Expression

    Bacterial and Plant

    Mutation

    The Arabidopsis thaliana U5 small nuclear ribonuc…

    Promoter

    35S promoter, Hordeum vulgare HSP 17 promoter use…

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNvT-MHC::mCh

    Plasmid

    #67943

    Purpose

    Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

    Depositor

    Ulrich Technau

    Article

    Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    mCherry
    

    Use

    Transgenic reporter in the sea anemone, nematoste…

    Tags

    Expression

    Mutation

    Promoter

    Myosin Heavy Chain1 (MyHC1)

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDS221

    Plasmid

    #34981

    Purpose

    Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb1–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P1-3xMBT

    Plasmid

    #46987

    Purpose

    Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

    Depositor

    Or Gozani

    Article

    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

    Insert

    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    

    Use

    Tags

    GST and PreScission cleavage site

    Expression

    Bacterial

    Mutation

    contains amino acids 190–530 of accession NP_0562…

    Promoter

    tac

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57KAN-40XALFA

    Plasmid

    #233118

    Purpose

    40X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57KAN-20XALFA

    Plasmid

    #233112

    Purpose

    20X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3 Promoter Vector

    Plasmid

    #212939

    Purpose

    Control vector with constitutive firefly luciferase expression

    Depositor

    Debrya Groskreutz

    Insert

    luc+
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    SV40

    Available Since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM5188

    Plasmid

    #120087

    Purpose

    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFP-mTet1s pc3901

    Plasmid

    #201700

    Purpose

    Expresses of GFP-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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