We narrowed to 3,403 results for: aaas

1. pPBO.ACT005

   Plasmid

   #182715

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-SETin-FLAG-HA

   Plasmid

   #24999

   Depositor

   Judy Lieberman

   Article

   Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

   Insert

   SET (SET Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   silent mutations introduced to prevent siRNA targ…

   Available Since

   Aug. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

3. Lenti_CRISPRi_sgNeg1

   Plasmid

   #166998

   Purpose

   Lentiviral expression of negative control sgRNA for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   Negative control sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2SCb

   Plasmid

   #59385

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat2
   

   Use

   Template

   Mutation

   The 3’-end of the chloramphenicol resistance gene…

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 shTGFBR3 puro

   Plasmid

   #58696

   Purpose

   Lentiviral shRNA vector for knockdown of human TGFBR3

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   shTGFBR3 (TGFBR3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

7. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_TFE3_2

   Plasmid

   #86326

   Purpose

   Encodes gRNA for 3' target of human TFE3

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against TFE3 (TFE3 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Oct4-Cr1

   Plasmid

   #66989

   Purpose

   Expresses gRNA that targets stop codon for OCT4

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   gRNA for Crispr targeting the OCT4 stop codon
   

   Expression

   Mammalian

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

10. SL2-sgSat-MTSb/BFP/pdCas9-C1

    Plasmid

    #162761

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting centromeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgSat-MTSb)
    

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1P shABCB7_2

    Plasmid

    #102969

    Purpose

    Suppression of ABCB7

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shABCB7_2 (ABCB7 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

    Plasmid

    #114729

    Purpose

    3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M5, sgRNA M7, sgRNA M9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-OSBP

    Plasmid

    #32495

    Depositor

    Alex Toker

    Article

    Nhek et al Mol Biol Cell. 2010 Jul 1;21(13):2327-37

    Insert

    OSBP (OSBP Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pWL502-crRNA

    Plasmid

    #174383

    Purpose

    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    a crRNA targeting the template strand
    

    Use

    CRISPR; Archaeal expression

    Expression

    Bacterial

    Promoter

    PhaR promoter

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-sh-mSox10-2

    Plasmid

    #37007

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSox10-2 (Sox10 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pLenti CRISPR sgSHMT1_1

    Plasmid

    #106311

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSico-shRheb

    Plasmid

    #81087

    Purpose

    conditional expression of an shRNA targeting murine Rheb

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    Rheb shRNA (Rheb Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTC365

    Plasmid

    #91214

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSiren_EGFP-shHmga1#1

    Plasmid

    #122288

    Purpose

    Knockdown for mouse Hmga1

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Insert

    Knockdown shRNA for mouse Hmga1 (Hmga1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1 puro shPRDM14-2

    Plasmid

    #193695

    Purpose

    Constitutive lentiviral expression of PRDM14 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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