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172,691 results

Showing: 2141 - 2160 of 172691 results

  2. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

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  4. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100854-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-DIO-ATLASsnFlp (AAV8)

    Viral Prep
    #232352-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-DIO-ATLASsnFlp (#232352). In addition to the viral particles, you will also receive purified pAAV-DIO-ATLASsnFlp plasmid DNA. Syn-driven, ATLASsnFlp expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Don Arnold
    Article
    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d
    Promoter
    Syn
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3

    Plasmid
    #172922
    Purpose
    For enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3
    Depositor
    GENIE Project
    Has Service
    AAV1 and AAV9
    Insert
    axon-jGCaMP8m-P2A-mRuby3
    Use
    AAV
    Tags
    GAP43 palmitoylation domain (N terminal on insert…
    Expression
    Mammalian
    Promoter
    hSynapsin1
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172922-plasmid-map-sequence-id-341476

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  11. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  12. CHRM2-DuET

    Plasmid
    #213208
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM2 (CHRM2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213208-plasmid-map-sequence-id-430770

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  14. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep
    #100845-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available Since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-466939

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  18. pTREtight2

    Plasmid
    #19407
    Depositor
    Markus Ralser
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Oct. 15, 2008
    Availability
    Academic Institutions and Nonprofits only
    19407-plasmid-map-sequence-id-181154

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Showing: 2141 - 2160 of 172691 results