164,728 results

1. pT7-7 asyn WT

   Plasmid

   #36046

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. dTomato-2xrGBD

   Plasmid

   #129625

   Purpose

   Visualization of active RhoA

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   dTomato-2xrGBD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVdel

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

   Plasmid

   #67981

   Purpose

   Cas9 activity reporter (control) with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX iLOV

   Plasmid

   #26587

   Depositor

   John Christie

   Article

   Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

   Insert

   iLOV
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

   Plasmid

   #207180

   Purpose

   Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CbS-Flag-TRRAP

   Plasmid

   #32103

   Depositor

   Michael Cole, Yardena Samuels

   Insert

   Transformation/transcription domain-associated protein (TRRAP Human)
   

   Use

   Tags

   Flag 1x

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 - TRC control

   Plasmid

   #10879

   Purpose

   Negative control shRNA vector containing non-hairpin insert.

   Depositor

   David Root

   Article

   Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

   Insert

   non-hairpin 18bp
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_COCVmut_G

   Plasmid

   #207262

   Purpose

   Mammalian expression of a mutant Cocal virus G protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   COCVmut_G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47A/Y209A/R354A; mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-jGCaMP8m-WPRE (AAV PHP.eB)

    Viral Prep

    #162375-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Tags

    None

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

12. pRSET iGluSnFR3 v857

    Plasmid

    #175186

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v857
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-Trastuzumab-IgA1/κ

    Plasmid

    #61888

    Purpose

    Expresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+HyPer7-NLS

    Plasmid

    #136468

    Purpose

    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Triple nuclear localization signal of SV40 (simia…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2/11 v1

    Plasmid

    #240486

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid protein

    Depositor

    Meghan Rego, Addgene Research Program

    Insert

    AAV2 Rep and AAV11 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid

    #72486

    Purpose

    Express Luc2 and tdTomato under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-N-Flag-NLRP3

    Plasmid

    #75127

    Purpose

    Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-mSA-EGFP-TM

    Plasmid

    #39863

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin, EGFP, TM helix
    

    Use

    Tags

    EGFP, FLAG, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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