We narrowed to 59,591 results for: uros

1. TET-pLKO.1 PURO shSMAD4 #1

   Plasmid

   #83089

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shSMAD4 (SMAD4, Smad4 Human, Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pWPI-IRES-Puro-Ak-TMPRSS2

   Plasmid

   #154986

   Purpose

   Lentiviral vector expressing Human TMPRSS2

   Depositor

   Sonja Best

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1 puro CXCR4 siRNA-1

   Plasmid

   #12271

   Depositor

   Bob Weinberg

   Article

   Orimo et al Cell. 2005 May 6. 121(3):335-48.

   Insert

   CXCR4 siRNA (CXCR4 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-mStayGold-Puro-CLTC

   Plasmid

   #227314

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMMP-MCS-IRES-Puro

   Plasmid

   #36952

   Depositor

   Bill Sugden

   Article

   Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-EEA-5g-OSK2M2L1-PGK-Puro

   Plasmid

   #102909

   Purpose

   PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

   Plasmid

   #105043

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stk11 (Stk11 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-puro/RAF1-S259A

   Plasmid

   #131727

   Purpose

   Mammalian Expression of RAF1

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S259A

   Promoter

   CMV

   Available Since

   May 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPR v2 puro hGSDME gRNA1

   Plasmid

   #223519

   Purpose

   knocking out hGSDME in human cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid

    #164637

    Purpose

    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

    Depositor

    Günter Schneider

    Insert

    target guide sequence PRMT5 inhibition (PRMT5 Human)
    

    Use

    CRISPR and Lentiviral; Interference

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-puro-mGSDME D270A

    Plasmid

    #223518

    Purpose

    Expresses mGSDME D270A in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (Gsdme Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    D270A

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-EMTB-TurboID-V5-puro

    Plasmid

    #190741

    Purpose

    A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    EMTB-TurboID-V5 (RPS14 Human, EMTB is human ensconsin; TurboID is engineered BirA from E.coli)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB700-mouse-ACTC1-Puro

    Plasmid

    #128326

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe(puro) mouse Egr2

    Plasmid

    #108097

    Purpose

    Retroviral vector for expression of mouse Egr2

    Depositor

    Peter Johnson

    Article

    Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

    Insert

    Egr2 (Egr2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMuLE ENTR SV40 PuroR L3-L2

    Plasmid

    #62164

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Puromycin
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-FNLSHiFiNG-P2A-Puro

    Plasmid

    #136903

    Purpose

    Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFiNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Mutation

    R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-Puro-mRuby-Sec23A

    Plasmid

    #36158

    Depositor

    David Stephens

    Article

    Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

    Insert

    Sec23A (SEC23A Human)
    

    Use

    Lentiviral

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

    Plasmid

    #102797

    Purpose

    Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Inserts

    sgRNA
    EGFP
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    H231L

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPuro3.1(+)_Strep-Lrrk2-Myc

    Plasmid

    #161583

    Purpose

    expression of full-length mouse Lrrk2 with C-terminal Myc tag

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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