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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7

Plasmid

#167266

Purpose

Overexpression of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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lenti-mito-mTagBFP2

Plasmid

#216130

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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FUGW-ER-TurboID

Plasmid

#200965

Purpose

3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

ER-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAF0688 Kp03_attP-mCherry donor

Plasmid

#193467

Purpose

Promoterless mcherry donor for Kp03

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Golgi-TurboID

Plasmid

#200959

Purpose

Transiently expresses golgi localized TurboID with HA tags

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-Golgi-TurboID

Plasmid

#200964

Purpose

3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-Cytosol-TurboID

Plasmid

#200961

Purpose

3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Cytosol-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJBL7085

Plasmid

#167249

Purpose

orthogonal mRFP reporter output for cell-free expression

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-mRFP1

Use

Synthetic Biology

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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p1A

Plasmid

#162692

Purpose

Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL7086

Plasmid

#167250

Purpose

orthogonal Mango reporter output for cell-free expression

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-Mango

Use

Synthetic Biology

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFE-Reu-FI-prk

Plasmid

#162696

Purpose

Expresses R. eutropha Form IC rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

R. eutropha CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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B52 (empty plasmid backbone to express 2 sgRNAs)

Plasmid

#100708

Purpose

Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pGLOW30

Plasmid

#172697

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-2

Available Since

Sept. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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lenti-NLS-mTagBFP2

Plasmid

#216128

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone1

Plasmid

#162125

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJL056

Plasmid

#177168

Purpose

Expresses wildtype MRE11 in human cells

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAF0451 Si74_attP-mCherry donor

Plasmid

#193470

Purpose

Promoterless mcherry donor for Si74

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Si74_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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amilCP chromoprotein

Plasmid

#117847

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAF0474 Ef1a-Si74_attB acceptor

Plasmid

#193475

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Si74_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1_His-TEV-EGFP

Plasmid

#223723

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

EGFP, not monomeric (has A207)

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS1011

Plasmid

#238212

Purpose

Expresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

ParTasR

Tags

HA-NLS

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pFE-Rs-FI-prk

Plasmid

#162697

Purpose

Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

R. spaeroides Form IC rubisco and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Has Service

AAV6 and AAV8

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1

Plasmid

#13798

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pAF0690 Ef1a-Kp03_attB-BFP acceptor

Plasmid

#193473

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PB_iMYOD1_P2A_GFP_Puro

Plasmid

#168804

Purpose

PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

MYOD1 (MYOD1 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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