We narrowed to 8,835 results for: sgRNA

1. pLY130

   Plasmid

   #130952

   Purpose

   Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Anderson promoter J23100

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLY106

   Plasmid

   #130941

   Purpose

   Engineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEA4
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plux2

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAK010

   Plasmid

   #128227

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 4

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAK011

   Plasmid

   #128228

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 5

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 5
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAK012

   Plasmid

   #128229

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 6

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 6
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH87

   Plasmid

   #128200

   Purpose

   sgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   sgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPOR1CB0022

   Plasmid

   #117537

   Purpose

   Level1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   sgRNA (Sp Cas9) - extended stem
   

   Expression

   Plant

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWT060b

   Plasmid

   #107904

   Purpose

   W2m.0-2 plasmid. Contains U6-sgRNA A (CCR5) and is part of a system for CAMERA 2.5

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   U6-sgRNA A (CCR5) (CCR5 Human)
   

   Expression

   Mammalian

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-AmCyan

   Plasmid

   #138481

   Purpose

   For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   AmCyan
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

    Plasmid

    #138482

    Purpose

    Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Ribozyme-flanked gRNA and AmCyan
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDYT001

    Plasmid

    #186549

    Purpose

    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs)

    Depositor

    Jonathan Weissman

    Article

    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

    Insert

    14-bp random integration barcode and three target sites and 3x sgRNAs
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PL-5LTR-GW-A

    Plasmid

    #138480

    Purpose

    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-U6-sgMettl3

    Plasmid

    #196263

    Purpose

    Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

    Depositor

    Laura Attardi

    Article

    Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

    Insert

    Mettl3 sgRNA (Mettl3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. peSCBE3-NG-HF1-Hypa

    Plasmid

    #218166

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGRACE

    Plasmid

    #230910

    Purpose

    Tracing of CRISPR activity in whole animals.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    GFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEE082 FoMV

    Plasmid

    #234811

    Purpose

    To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS159

    Plasmid

    #122378

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJPC596 FoMV-AmCyan

    Plasmid

    #234812

    Purpose

    To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    AmCyan
    

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    Eric Lander, David Sabatini

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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