We narrowed to 5,055 results for: cre encoding plasmid

1. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

   Plasmid

   #169187

   Purpose

   To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOGS539_haPD-1

   Plasmid

   #226716

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   High affinity PD-1 microbody
   

   Use

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOGS539_rPD-L1nb

   Plasmid

   #226717

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Use

   Tags

   HA tag, Histidine tag, and Mating factor alpha se…

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-SARS2-S-G614 (C-flag)

   Plasmid

   #156421

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. K84M kinase-dead Nuak1-3xflag

   Plasmid

   #97219

   Purpose

   Plasmid expressing kinase-dead mutant Nuak1

   Depositor

   Huda Zoghbi

   Article

   Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

   Insert

   Nuak1 (NUAK1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Kinase-dead K84M

   Promoter

   T7

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-SARS2-S-D614 (C-flag)

   Plasmid

   #156420

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCOLADuet-1_nsp10 (SARS-CoV-2)

   Plasmid

   #169158

   Purpose

   To express SARS-CoV-2 nsp10 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-noSV40-humanCOL2A1enhancer

   Plasmid

   #173961

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Enhancer region from COL2A1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-SARS2-S-D614 (NC-flag)

   Plasmid

   #156418

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-noSV40-humanEC1.45

    Plasmid

    #173946

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid

    #159364

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid

    #159363

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Use

    Tags

    Flag and Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid

    #156419

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

    Plasmid

    #169166

    Purpose

    Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

    Insert

    3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

    Plasmid

    #226989

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 1a to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

    Plasmid

    #226991

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 2a to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid

    #226992

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 2b to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

    Plasmid

    #226990

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 1b to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV Retrograde)

    Viral Prep

    #105558-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Tags

    None

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

20. AAV-pgk-Cre (AAV Retrograde)

    Viral Prep

    #24593-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV-pgk-Cre (#24593). In addition to the viral particles, you will also receive purified AAV-pgk-Cre plasmid DNA. Cre expression under the PGK promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.

    Depositor

    Patrick Aebischer

    Promoter

    pgk

    Tags

    None

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only