We narrowed to 3,461 results for: mpo

1. pEGFP-C1-Cep123 (CCDC123/Cep89)

   Plasmid

   #67787

   Purpose

   This plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

   Insert

   Cep123 (CEP89 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLKO.1P POLE1_1

   Plasmid

   #160762

   Purpose

   Suppress POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLE1_1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAL113

   Plasmid

   #22471

   Purpose

   Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB NT (PHYB Mustard Weed, Mouse)
   

   Use

   Synthetic Biology

   Tags

   10aaLinker-mCFP-HrasCT

   Expression

   Mammalian

   Mutation

   Y276H

   Promoter

   CMV

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. anxA10-GFP

   Plasmid

   #107199

   Purpose

   Expresses human annexin A10-GFP fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

   Insert

   annexin A10 (ANXA10 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCK314

   Plasmid

   #110545

   Purpose

   Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

   Inserts

   Modified PrhaBAD (CRP-binding sites removed)
   yfp
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PDZb-beta2(616-951)-mCherry

   Plasmid

   #100736

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   PDZb and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FastLightR-bRaf-mVenus

   Plasmid

   #162155

   Purpose

   Expresses FastLightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   FastLightR-bRaf-mVenus
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPonA-BI-Gl NORM-LacZA TER- LacZB

   Plasmid

   #86212

   Purpose

   beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter

   Depositor

   Robert Singer

   Article

   Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

   Insert

   beta-Globin (HBB, Hbb Human, Mouse)
   

   Use

   Ponasterone a inducible promoter

   Tags

   lacZA or lacZB

   Expression

   Mammalian

   Mutation

   39TER in beta Globin in MCS II

   Promoter

   Ponasterone A inducible

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. prk5-MFSD12-TEV-3xFLAG-3xHA

    Plasmid

    #149504

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRL838

    Plasmid

    #70680

    Purpose

    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

    Depositor

    Peter Wolk

    Article

    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMyc-C1-Cep123 (isoform 3)

    Plasmid

    #73328

    Purpose

    Encodes isoform 3 of Cep123 fused to a myc tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

    Insert

    Cep123 (CEP89 Human)
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-soCoChR-GFP

    Plasmid

    #107710

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mcherry Gamma 9 ---pcDNA3.1

    Plasmid

    #64203

    Purpose

    G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    G protein Gamma 9 (GNGT2 Human)
    

    Use

    Tags

    6xHis and mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLKO.1P POLE1_2

    Plasmid

    #160764

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-pmEMBer

    Plasmid

    #174441

    Purpose

    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEMBer
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMyc-C1-Cep123 (isoform 2)

    Plasmid

    #73327

    Purpose

    Encodes isoform 2 of Cep123 (Cep89/CCDC123) fused to a myc tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

    Insert

    Cep123 (CEP89 Human)
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-Puro Xlone SOX17

    Plasmid

    #179838

    Purpose

    Doxycycline-inducible expression of human SOX17

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    AAV, CRISPR, Synthetic Biology, and TALEN ; Donor…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge