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Showing: 401 - 440 of 1185 results

  2. bbCas9pluspAAA

    Plasmid
    #82581
    Purpose
    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA
    Depositor
    Ivo Huijbers
    Article
    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:
    Insert
    Cas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    82581-plasmid-map-sequence-id-228465

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  12. pBTSG

    Plasmid
    #159420
    Purpose
    Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells
    Depositor
    Dianfan Li
    Article
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Use
    Tags
    polyhistidine (poly-His)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159420-plasmid-map-sequence-id-321535

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  15. pETSG

    Plasmid
    #159418
    Purpose
    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli
    Depositor
    Dianfan Li
    Article
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Use
    Tags
    polyhistidine (poly-His)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159418-plasmid-map-sequence-id-321533

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  28. pYTSG

    Plasmid
    #159419
    Purpose
    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae
    Depositor
    Dianfan Li
    Article
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Use
    Tags
    polyhistidine (poly-His)
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159419-plasmid-map-sequence-id-321534

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  32. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid
    #99696
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Afp (Afp Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    99696-plasmid-map-sequence-id-194593

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  33. pHIT239

    Plasmid
    #204522
    Purpose
    In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematode
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    Mos1_transposase::glh-2_3'UTR (glh-2 Nematode)
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    T7 (minimum seq.)
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204522-plasmid-map-sequence-id-403812

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  40. c-Flag pcDNA3

    Plasmid
    #20011
    Purpose
    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.
    Depositor
    Stephen Smale
    Article
    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20011-plasmid-map-sequence-id-372933

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Showing: 401 - 440 of 1185 results