We narrowed to 46,724 results for: Eng;

1. pBbA5c-MevT-MBI (JBEI-3085)

   Plasmid

   #87950

   Purpose

   the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   MevT-MBI
   

   Expression

   Bacterial

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPMVAu8

   Plasmid

   #98297

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
   

   Expression

   Yeast

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas2C

   Plasmid

   #82374

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   PEZ3

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ARK2aa

   Plasmid

   #81013

   Purpose

   E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)

   Depositor

   Taek Soon Lee

   Article

   Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

   Inserts

   MevTo-BBa1002-pTrc-Mkco-aphA
   and PMD
   

   Promoter

   LacUV5 and Trc promoter

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTCW012

   Plasmid

   #83527

   Purpose

   Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   pFUS1J2

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCORE-Kp53

   Plasmid

   #72241

   Purpose

   Contains the CORE cassette with KanMX4 and human p53 gene markers

   Depositor

   Francesca Storici, Michael Resnick

   Article

   Storici et al Genet Eng (N Y). 2003;25:189-207.

   Inserts

   p53 (TP53 Human)
   kanMX4
   

   Expression

   Bacterial

   Mutation

   V122A mutation

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-Casp2

   Plasmid

   #50023

   Purpose

   pENTR vector for mouse Caspase-2

   Depositor

   Jamie Davies

   Article

   Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

   Insert

   Casp2 (Casp2 Mouse)
   

   Use

   Entry vector

   Available Since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCORE-Hp53

   Plasmid

   #72242

   Purpose

   Contains the CORE cassette with hyg and human p53 gene markers

   Depositor

   Francesca Storici, Michael Resnick

   Article

   Storici et al Genet Eng (N Y). 2003;25:189-207.

   Inserts

   p53 (TP53 Human)
   hyg
   

   Expression

   Bacterial

   Mutation

   V122A mutation

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas5

   Plasmid

   #82378

   Purpose

   sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23119

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbE1k-2

    Plasmid

    #59945

    Purpose

    dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase
    pterin-4a-carbinolamine dehydratase
    

    Promoter

    pTrc

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas2F

    Plasmid

    #82377

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pChlr-7SE

    Plasmid

    #52168

    Purpose

    Encodes module 7 with amino acids 12S-16E for recognition of nt 2G

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pChlr-4SYE

    Plasmid

    #52156

    Purpose

    Encodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    D43951 (PUM1 Human)
    

    Expression

    Bacterial

    Mutation

    Changed 12N to 12S, 13H to 13Y, and 16Q to 16E in…

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pChlr-3CQ

    Plasmid

    #52147

    Purpose

    Encodes module 3 with amino acids 12C-16Q for recognition of nt 6A

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pChlr-1SQ

    Plasmid

    #52139

    Purpose

    Encodes module 1 with amino acids 12S-16Q for recognition of nt 8A

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pChlr-2NQ

    Plasmid

    #52145

    Purpose

    Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pChlr-4NQ

    Plasmid

    #52153

    Purpose

    Encodes module 4 with amino acids 12N-16Q for recognition of nt 5U

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pChlr-5CQ

    Plasmid

    #52159

    Purpose

    Encodes module 5 with amino acids 12C-16Q for recognition of nt 4A

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pChlr-6NQ

    Plasmid

    #52165

    Purpose

    Encodes module 6 with amino acids 12N-16Q for recognition of nt 3U

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pChlr-8NQ

    Plasmid

    #52173

    Purpose

    Encodes module 8 with amino acids 12N-16Q for recognition of nt 1U

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    

    Expression

    Bacterial

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCas2D

    Plasmid

    #82375

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCas61

    Plasmid

    #82397

    Purpose

    IPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpK

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    LDH
    

    Mutation

    V39R

    Promoter

    cLac143

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEMY07AB-PRO

    Plasmid

    #127710

    Purpose

    Level 0 destination vector for a promoter with A - B scars. Clone in with BpiI

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCas2B

    Plasmid

    #82373

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCas8

    Plasmid

    #82380

    Purpose

    dCas9 without a promoter in acsA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    none

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCas7

    Plasmid

    #82379

    Purpose

    dCas9 expressed from low constituve promoter, c225, in acsA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    c225

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCas2E

    Plasmid

    #82376

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCas2A

    Plasmid

    #82372

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCas28

    Plasmid

    #82385

    Purpose

    sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23108

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCas27

    Plasmid

    #82384

    Purpose

    sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23117

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ARK2a

    Plasmid

    #81017

    Purpose

    E.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco
    PMD
    

    Promoter

    LacUV5 and Trc promoter

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ARK1a

    Plasmid

    #81016

    Purpose

    E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco-PMKco
    PMD-NudB
    

    Promoter

    LacUV5 and Trc promoter

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEMY07AB-ORF

    Plasmid

    #127711

    Purpose

    Level 0 destination vector for a coding sequence with B - C scars. Clone in with BpiI

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEMY07CD-TER

    Plasmid

    #127712

    Purpose

    Level 0 destination vector for a terminator with C - D scars. Clone in with BpiI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDSM-5a

    Plasmid

    #127716

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 1. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDSM-ab

    Plasmid

    #127717

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 2. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDSM-bc

    Plasmid

    #127718

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 3. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDSM-cd

    Plasmid

    #127719

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 4. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDSM-de

    Plasmid

    #127720

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDSM-ef

    Plasmid

    #127721

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDSM-f3

    Plasmid

    #127722

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCORE-UH

    Plasmid

    #72239

    Purpose

    Contains the CORE cassette with KlURA3 and hyg markers

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    KlURA3
    hyg
    

    Expression

    Bacterial

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCORE-UK

    Plasmid

    #72238

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    KlURA3
    kanMX4
    

    Expression

    Bacterial

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBEST-melR-deGFP-T500

    Plasmid

    #45396

    Depositor

    Vincent Noireaux

    Article

    Shin et al J Biol Eng. 2010 Jun 24;4:8.

    Insert

    melibiose repressor
    

    Expression

    Bacterial

    Available Since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBADmod1-linker-deGFP

    Plasmid

    #45397

    Depositor

    Vincent Noireaux

    Article

    Shin et al J Biol Eng. 2010 Jun 24;4:8.

    Insert

    deGFP
    

    Expression

    Bacterial

    Available Since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

47. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJT1

    Plasmid

    #98299

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
    

    Expression

    Yeast

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCN-mBFP

    Plasmid

    #63847

    Purpose

    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

    Depositor

    Howard Salis

    Article

    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

    Insert

    mBFP
    

    Expression

    Bacterial

    Mutation

    Promoter and RBS was swapped. His-tag was removed…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pPMVAd3

    Plasmid

    #98298

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
    

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge