We narrowed to 30,491 results for: Ide
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Plasmid#162683PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationS136C, G489C, S530C, and a sequence Y75-F104 to i…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Antibody#218106-rAbPurposeAnti-DYKDDDDK (Flag) tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds in a calcium-dependent manner to the N-terminal DYKDDDDK sequence.DepositorRecommended ApplicationsWestern BlotReactivitySyntheticSource SpeciesRabbitIsotypeIgG1Trial SizeNot available to purchaseAvailable sinceMay 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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Antibody#218108-rAbPurposeAnti-Protein C tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to EDQVDPRLIDGK sequence.DepositorRecommended ApplicationsWestern BlotReactivityHumanSource SpeciesRabbitIsotypeIgG1Trial SizeNot available to purchaseAvailable sinceMay 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pJK034
Plasmid#72383PurposeProduces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)DepositorInsertphosphoribosylglycinamide synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Yeast Secrete and Detect
Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Start-Stop Assembly Toolkit
Plasmid Kit#1000000152PurposeStart-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.DepositorAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_T1-NHis
Plasmid#109259PurposeNRPS T domain with N-terminal 6His tagDepositorArticleInsertRupA_T1
UseTagsHis tagExpressionBacterialMutationcontains aa 917..1025 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_R-NHis
Plasmid#109257PurposeNRPS R domain with N-terminal 6His tagDepositorArticleInsertRupA_R
UseTagsHis tagExpressionBacterialMutationcontains aa 2038..2443 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJQ210SK
Plasmid#78499PurposeCosmid version of suicide vector pJQ200SK. Suicide vectorDepositorTypeEmpty backboneUseSuicide vectorTagsnoneExpressionMutationPromoternonAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_T2-R-CHis
Plasmid#109258PurposeNRPS di-domain containing TR domains with C-terminal 6His tagDepositorArticleInsertRupA_T2-R
UseTagsHis tag and S-TagExpressionBacterialMutationcontains aa 1939..2443 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_C2-A2-T2-R-CHis
Plasmid#109256PurposeNRPS module containing CATR domains with C-terminal 6His tagDepositorArticleInsertRupA_C2-A2-T2-R
UseTagsHis tag and S-TagExpressionBacterialMutationcontains aa 1023..2443 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_C1-A1-T1-CHis
Plasmid#109255PurposeNRPS module containing CAT domains with C-terminal 6His tagDepositorArticleInsertRupA_C1-A1-T1
UseTagsHis tag and S-TagExpressionBacterialMutationcontains aa 1..1025 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)
Plasmid Kit#1000000221PurposeA positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.DepositorAvailable sinceJune 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKB225
Plasmid#170015PurposeEncodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Peptide display protein with no C-terminal peptide
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
iRFP-PH-PLCdelta1
Plasmid#66841Purposeexpression of near-infrared PI(4,5)P2 biosensorDepositorInsertPH-PLCdelta1
UseTagsiRFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-SwiChRca-TS-EYFP
Plasmid#55630PurposeChloride ChannelDepositorInsertiC1C2-TS-EYFP
UseAAVTagsEYFPExpressionMammalianMutationC128APromoterCaMKIIaAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
NM9-SpCas9-NLS3
Plasmid#128177PurposeExpresses R. toruloides codon-optimized SpCas9 under PGK1 promoterDepositorInsertSpCas9-NLS3
UseCRISPRTagsExpressionYeastMutationPromoterpPGK1Available sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
NM1-5S-tRNA-SgH
Plasmid#128178PurposeExpresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoterDepositorInsertgRNA cloning cassette
UseCRISPRTagsExpressionYeastMutationPromoter5S rRNA-tRNA(Arg)Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrCAB
Plasmid#174618PurposeBacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.DepositorInsertmtrCAB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLacIAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only