We narrowed to 25,308 results for: Nes

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pAAV.CAG.LSL.tdTomato

Plasmid

#100048

Purpose

AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdtomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA HA DUSP6

Plasmid

#236430

Purpose

transient overexpression of DUSP6 in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

Insert

DUSP6 (DUSP6 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215326

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

dNTP63 Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

dNTP63

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGES201

Plasmid

#190197

Purpose

For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

Depositor

Yuefeng Guan

Article

Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

Insert

spCas9

Use

CRISPR

Tags

3xFlag

Expression

Plant

Mutation

plant-codon optimized

Promoter

Glycine max elongation factor 1A(pM4)

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-3xFLAG-hSpCas9

Plasmid

#162161

Purpose

Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

3xFLAG

Expression

Insect

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.LSL.EGFP

Plasmid

#100047

Purpose

AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF16B tail 458-1317

Plasmid

#134624

Purpose

Mammalian expression of human KIF16B 458-1317

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF16B (KIF16B Human)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 457 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO

Plasmid

#73311

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pG3H-PE3max-attR1R2

Plasmid

#213049

Purpose

Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Inserts

Cas9 nickase
M-MLV Reverse Transcriptase

Use

Note: this plasmid needs helper pvs1-vir2 for rep…

Expression

Plant

Mutation

H840A, R240K, N413K,

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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KIF1A(1-393)-GCN4-2xmCherry-EF(C)

Plasmid

#61665

Purpose

Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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TRRAP sgRNA3

Plasmid

#138184

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre-T2A-mRuby2

Plasmid

#102989

Purpose

Expresses Cre and mRuby2

Depositor

Karin Scharffetter-Kochanek

Article

Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

Inserts

Cre (site-specific recombinase)
mRuby2

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-3xT7-CA-ChREBP

Plasmid

#171029

Purpose

Expresses 3xT7-tagged constitutive active form of ChREBP in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Insert

CA-ChREBP (Mlxipl Mouse)

Use

Retroviral

Tags

3xT7

Expression

Mammalian

Mutation

amino acids 240 - 864

Promoter

5' LTR

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9-pAc

Plasmid

#162163

Purpose

Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

T2A-pAc

Expression

Insect

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13A tail 361-1749

Plasmid

#134622

Purpose

Mammalian expression of mouse KIF13A 361-1749

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13A (Kif13a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 360 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13B tail 442-1843

Plasmid

#134623

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 441 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EFIa-Id1-PGK-puro

Plasmid

#107735

Purpose

Expresses Id1 in mammalian cells

Depositor

Alexandre Colas

Article

Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

Insert

Id1 (T Mouse)

Use

Lentiviral

Promoter

EF1a

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM22082_cRed

Plasmid

#117225

Purpose

pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

Insert

Canthaxanthin Operon

Tags

34 amino acid N-terminal linker, Hexahistidine Ta…

Expression

Bacterial

Mutation

removed Type IIS restriction sites within the bac…

Promoter

T7 RNA Polymerase

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO PPM1H

Plasmid

#207333

Purpose

Bacterial expression of His SUMO PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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