Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 14,447 results for: cas9 genes

Showing: 2181 - 2200 of 14447 results

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH

Plasmid

#148850

Purpose

Mammalian Expression of HsTNRC6B-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-sgRNA1

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH

Plasmid

#148851

Purpose

Mammalian Expression of HsAGO2-sgRNA2

Depositor

Elisa Izaurralde

Insert

HsAGO2-sgRNA2 (AGO2 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

Plasmid

#148852

Purpose

Mammalian Expression of HsTNRC6A-sgRNA2

Depositor

Elisa Izaurralde

Insert

HsTNRC6A-sgRNA2

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

Plasmid

#148853

Purpose

Mammalian Expression of HsTNRC6B-sgRNA3

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-sgRNA3

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

Plasmid

#148854

Purpose

Mammalian Expression of HsAGO1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO1-sgRNA (AGO1 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

Plasmid

#148856

Purpose

Mammalian Expression of HsNot1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsNot1-sgRNA (CNOT1 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

Plasmid

#148857

Purpose

Mammalian Expression of HsPatL1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsPatL1-sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

Plasmid

#148858

Purpose

Mammalian Expression of HsTNRc6C-sgRNA

Depositor

Elisa Izaurralde

Insert

HsTNRc6C-sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna2

Plasmid

#192796

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna2

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna6

Plasmid

#192797

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna6

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna6

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_1

Plasmid

#190703

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 1

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_3

Plasmid

#190705

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 3

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_1

Plasmid

#190700

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 1

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_2

Plasmid

#190701

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 2

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_3

Plasmid

#190702

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 3

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-H2BC11_sgRNA

Plasmid

#183881

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-MYH10_sgRNA

Plasmid

#183886

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_164 SpCas9 R1333A/R1335A

Plasmid

#179528

Purpose

For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 R1333A/R1335A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginines 1333 and 1335 to alanine

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Showing: 2181 - 2200 of 14447 results

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