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pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT WT

Plasmid

#154282

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Tags

2x FLAG and mKate2

Mutation

WT OGT

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Tags

Hexa his

Expression

Insect

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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yHS1-M7A

Plasmid

#159151

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1-M7A

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of yHS1 to A…

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCAD39_mIRG1_4-462_pvp008

Plasmid

#124873

Purpose

Expresses mouse CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (Acod1 Mouse)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 463-end.

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluSnFR

Plasmid

#106123

Purpose

Indicator for glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Tags

Myc and PDGFR

Expression

Mammalian

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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mEos3-dnRac1

Plasmid

#92040

Purpose

Expresses mEos3 fusion with dnRac1 (T17N).

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

T17N (dominant negative)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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mEos3-Rac1

Plasmid

#92039

Purpose

Expresses mEos3-Rac1 fusion protein

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-282-LUC

Plasmid

#46865

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Promoter

24p3 minimal promoter

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA1-Bystin

Plasmid

#11869

Depositor

Michiko Fukuda

Article

Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

Insert

Bystin (BYSL Human)

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pFRLuc-CoV2-WT

Plasmid

#177614

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-U63-gRNA2.1

Plasmid

#170512

Purpose

gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/avitag-apex2-v5-fkbp8

Plasmid

#154460

Purpose

expresses avitag-apex2-v5-fkbp8/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

avitag-apex2-v5-fkbp8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pKV015

Plasmid

#64766

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO2-Flp

Plasmid

#149296

Purpose

Cre-dependent expression of Flp recombinase.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

Flp recombinase (flp Budding Yeast)

Use

AAV

Mutation

Derived from the mouse codon-optomized FlpO. The…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBP-split-intein-Gal4[21-881]-C-int

Plasmid

#199202

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BLLF1(gp350)

Plasmid

#195837

Purpose

Gateway Entry Clone of Epstein-Barr virus BLLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BLLF1 (BLLF1 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pUAS-PE2-attB

Plasmid

#149550

Purpose

Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Expression

Insect

Promoter

UAS

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd

Plasmid

#135518

Purpose

Mammalian expression of myc-tagged H2-Dd

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

C121S

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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