We narrowed to 24,829 results for: cas9 genes

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pAAV-TRE-DIO-Cas9

Plasmid

#166601

Purpose

Encodes Cre-dependent Cas9 under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

Cas9

Use

AAV

Promoter

tetracycline response element

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-HF-nCas9

Plasmid

#163548

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-E2-ABE-Cas9n-puro

Plasmid

#226587

Purpose

Express E2-ABE in mammalian cells

Depositor

Gang Wang

Article

Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

Insert

E2-ABE

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti_PGK-Cas9-P2A-HygR

Plasmid

#164135

Purpose

Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHDE-35S-Cas9-mCherry

Plasmid

#78931

Purpose

For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

Depositor

Yunde Zhao

Article

Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pME-dCas9-BFP-KRAB

Plasmid

#221502

Purpose

CRISPRi machinery fish codon optimized

Depositor

Alessandro Ori

Insert

dCas9-tagBFP-KRAB

Use

Fish expression

Mutation

D10A and H839A point mutations

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin sgRNA

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pKMD106e - intein-Cas9(S219)

Plasmid

#64190

Purpose

Expresses intein-Cas9(S219) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-dCas9-KOX1

Plasmid

#240528

Purpose

Expresses MMLVgag–dCas9-KOX1 for producing RENDER-dCas9-KOX1

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-dCas9-KOX1

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-nCas9(D10A)-PmCDA1

Plasmid

#79617

Purpose

Expresses nCas9(D10A)-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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p-dCas9-HDAC1-H141A-Hygro

Plasmid

#104417

Purpose

transient expression of dCas9-dHDAC1 fusion protein

Depositor

Stephan Beck

Insert

dHDAC1 (HDAC1 Human)

Use

CRISPR

Expression

Mammalian

Mutation

H141A

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCF287_EFS-TiCas9-P2A-Puro

Plasmid

#211646

Purpose

EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Tamoxifen-inducible SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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LLP249 pGK-dCas9-DNMT3a V3

Plasmid

#100938

Purpose

dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)

Use

CRISPR and Lentiviral

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pspCas9-HF-tetra-com-vector

Plasmid

#132555

Purpose

Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

High fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMCS-rybozyme-IRES-CAS9

Plasmid

#64668

Purpose

Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

Depositor

Wataru Fujii

Article

Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

Inserts

CAS9
HDV ribozyme

Use

CRISPR

Tags

Flag, Internal ribosome entry site, and guide RNA…

Expression

Mammalian

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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xCas9(3.6)-ABE(7.10)

Plasmid

#108387

Purpose

Mammalian expression vector for xCas9(3.6)-ABE

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-ABE(7.10)

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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