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GGE258

Plasmid

#120775

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T3_Xpr2

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy52

Plasmid

#122006

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal His6-3xAvi with PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-3xAvi

Expression

Bacterial

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-c2G

Plasmid

#75290

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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BCJJ223

Plasmid

#117509

Purpose

AtMGE1 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pMGE1:AATG:BsaI

Expression

Plant

Promoter

AtMGE1

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL12021

Plasmid

#117511

Purpose

AtICU2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pICU2:AATG:BsaI

Expression

Plant

Promoter

AtICU2

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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p201N 1509

Plasmid

#55768

Purpose

Contains soybean miRNA miR1509 recognition sequence (CAACCTTGATTTCCTTGATTAA) to produce siRNAs from 3' target sequences and induce RNA silencing

Depositor

Wayne Parrott

Article

Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

Type

Empty backbone

Use

RNAi

Promoter

GmUbi

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pFF19H

Plasmid

#49783

Purpose

expresses hygromycin resistance gene in plant cells

Depositor

Joachim Messing

Article

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Insert

HPH

Use

Plant expression

Promoter

CaMV 35S

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-Gr64f

Plasmid

#21080

Depositor

Craig Montell

Article

Jiao et al Curr Biol. 2008 Nov 25. 18(22):1797-801.

Insert

Gr64f (Gr64f Fly)

Expression

Insect

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
p1ChlEF

Plasmid

#237277

Purpose

Empty vector for L1 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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p1ChlFG

Plasmid

#237278

Purpose

Empty vector for L1 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pStx2Trigger

Plasmid

#216332

Purpose

T7 promoter driving expression of an Stx2 RNA trigger sequence to activate Stx2 toehold switch

Depositor

Mark Styczynski

Article

Zhang et al Nat Commun. 2021 Sep 29;12(1):5724. doi:

Insert

Stx2 RNA Trigger

Expression

Bacterial

Available Since

March 17, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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p2KanEG

Plasmid

#237279

Purpose

Empty vector for L2 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pDASH-DIS-α2 (MoClo)

Plasmid

#242524

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, compatible with MoClo, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC0_N-TurboID

Plasmid

#231261

Purpose

For expressing fusion proteins with N terminal TurboID for proximity labeling in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_N-TurboID

Plasmid

#231277

Purpose

For stably expressing fusion proteins with N terminal TurboID for proximity labeling in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-DARPin-SpyCatcher

Plasmid

#243754

Purpose

For affinity purification of E01 anti-EGFR DARPin fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

DARPin-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-Nb-SpyCatcher

Plasmid

#243752

Purpose

For affinity purification of anti-mouse MHC-II nanobody (Nb) fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

Nb-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-scFv-SpyCatcher

Plasmid

#243753

Purpose

For affinity purification of anti-HA scFv fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

scFv-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SNAP

Plasmid

#243741

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SNAP tag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SNAP

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SpyTag

Plasmid

#243739

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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