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pCoofy56

Plasmid

#122008

Purpose

Mammalian vector for parallel SLIC cloning containing N-terminal TwinStrep and PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10/none and TwinStrep

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2039

Plasmid

#39397

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-20-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-Backbone(NI)

Plasmid

#27962

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NI)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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p_SNIPR_target_WT

Plasmid

#139465

Purpose

T7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensor

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_WT

Expression

Bacterial

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Backbone(HD)

Plasmid

#27964

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(HD)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pAWp7

Plasmid

#72730

Purpose

pFUGW-UBCp-RFP-CMVp-GFP

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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p_SNIPR_target_mut_36

Plasmid

#139466

Purpose

T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

Target_mut36

Expression

Bacterial

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMetOYC-Dest

Plasmid

#105082

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning

Depositor

Christopher Grefen

Article

Xing et al Plant Physiol. 2016 Jun;171(2):727-58. d

Type

Empty backbone

Tags

Cub-PLV and mOST4

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEP-EGFPin

Plasmid

#60961

Purpose

PCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

eGFP-Kana_I-SceI

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182862

Purpose

Hetero-tetramer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEYFP-mCherry2-mEGFP-mApple

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEZ10

Plasmid

#81135

Purpose

pACSA_J23113_tetR PEZ3_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBLO5.01

Plasmid

#121418

Purpose

pUC19_BsaI-dCas9-BsaI

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

dcas9

Expression

Bacterial

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJK59-ATG17-GFP

Plasmid

#44175

Depositor

James Hurley

Article

Ragusa et al Cell. 2012 Dec 21;151(7):1501-12. doi: 1

Insert

Atg17 (ATG17 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

none

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
TAL2084

Plasmid

#39452

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-48-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pUC19/DdmEGFP

Plasmid

#154304

Purpose

cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mEGFP

Expression

Bacterial

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2052

Plasmid

#39410

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-27-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL2053

Plasmid

#39411

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-27-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pMOD_A5801

Plasmid

#127223

Purpose

35S::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#113252

Purpose

pUC19 backbone with E. coli CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

E. coli CRISPR array

Use

Integration substrate

Promoter

NA

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#127224

Purpose

CmYLCV::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge