We narrowed to 2,507 results for: dCas9

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pRS143

Plasmid

#122377

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

Plasmid

#127660

Purpose

Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Mutation

E225A/D227A

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTE5425

Plasmid

#229032

Purpose

Expression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP (D249E, catalytically inactive)

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 CI

Plasmid

#227191

Purpose

Inducible lentiviral expression of CI (Catalytically Inactive) APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

H309N

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

Plasmid

#211844

Purpose

Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA and mCherry

Expression

Plant

Mutation

C492A

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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GB4068

Plasmid

#187810

Purpose

Module for the constitutive expression of Cup2:Gal4AD and copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

copper-inducible dCasEV2.1

Expression

Plant

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-2

Plasmid

#109010

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #2 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgULK1-1

Plasmid

#109004

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ULK1-sgRNA #1 (ULK1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1056

Plasmid

#71824

Purpose

Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-dnGFP-OGT(13, K852A)

Plasmid

#247678

Purpose

Expresses catalytically inactive full-length OGT K852A mutant fused to a destabilized GFP nanobody

Depositor

Christina Woo

Article

Mody et al Cell Chem Biol. 2025 Dec 18;32(12):1486-

Insert

HA-dnGFP-OGT(13, K852A) (OGT Human)

Tags

HA-tag

Expression

Mammalian

Mutation

K852A mutation made in OGT

Promoter

T7

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Tags

6x His tag and SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAT15489_dABE8e

Plasmid

#174127

Purpose

plasmid expressing ABE8e with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

Plasmid

#104569

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x2)

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

Plasmid

#104568

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x1)

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

Plasmid

#208047

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Mutation

Cys>Ala mutations in conserved RING domain

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCR1003

Plasmid

#71821

Purpose

Express Streptococcus pyogenes dCas9 (D10A, H840A)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC446 - pAAV EF1a hPREP(S554A)

Plasmid

#59968

Purpose

An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Expression

Mammalian

Mutation

S554A

Promoter

EF1a

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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