We narrowed to 16,615 results for: puromycin

1. NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124289

   Purpose

   sgRNA against human NFATc4

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   NFATc4 nuclear factor of activated T cells (NFATC4 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

2. integrin alpha9 / alpha2 EGFP pBabe puro

   Plasmid

   #13606

   Depositor

   Dean Sheppard

   Insert

   integrin alpha 9 / alpha 2 (ITGA9 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Integrin alpha 9 chimera containing the cytoplasm…

   Available Since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pLV[TetOn]-Puro-TRE3G>mAscl1

   Plasmid

   #198755

   Purpose

   Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Promoter

   TRE3G

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCM-CLYBL-NGN2-BRN3A

   Plasmid

   #165597

   Purpose

   Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

   Depositor

   Carsten Bönnemann

   Article

   Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   BRN3A (POU4F1 Human)
   EGFP
   Puromycin N-acetyl transferase
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

   Available Since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV[TetOn]-Puro-TRE3G>mNeurog2

   Plasmid

   #198754

   Purpose

   Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Neurogenin-2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Promoter

   TRE3G

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV Puro DEST (w118-1) IGF1R

   Plasmid

   #107499

   Purpose

   Expresses IGF1R, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   IGF-1R (IGF1R Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV Puro DEST (w118-1) PHLDB1

   Plasmid

   #107503

   Purpose

   Expresses PHLDB1, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   PHLDB1 (PHLDB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Nucleotide changes 1236 T>C, 1365 C>T, 1371…

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV Puro DEST (w118-1) EPB41L1

   Plasmid

   #107508

   Purpose

   Expressing EPB41L1, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   EPB41L1 (EPB41L1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV Puro DEST (w118-1) FAM149A

    Plasmid

    #107510

    Purpose

    Expressing FAM149a, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    FAM149A (FAM149A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid

    #171803

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-gB-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

    Plasmid

    #171812

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-gB-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6.2_mNeonGreen2

    Plasmid

    #113727

    Purpose

    Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Article

    Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

    Insert

    mNeonGreen2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PLKO.1-Scrambled

    Plasmid

    #136035

    Purpose

    Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    None (Scrambled)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDRGFP

    Plasmid

    #26475

    Purpose

    In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker

    Depositor

    Maria Jasin

    Article

    Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.

    Insert

    DRGFP
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. scramble shRNA

    Plasmid

    #1864

    Purpose

    3rd gen lentiviral negative control vector containing scrambled shRNA

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDAC627

    Plasmid

    #195240

    Purpose

    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-CRISPR

    Plasmid

    #160047

    Purpose

    For cloning sgRNA to knockout a specific gene in piggybac plasmid

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. KW78_HART_ODC1_PuroR

    Plasmid

    #207401

    Purpose

    Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR - PuroR

    Depositor

    Stephen Floor

    Article

    Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mini CMV

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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