We narrowed to 8,407 results for: reporter

1. A3Bmax

   Plasmid

   #207167

   Purpose

   Express full-length human A3B in a BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
   

   Tags

   NLS and NLS, 6x His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

   Plasmid

   #202568

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB128

   Plasmid

   #205137

   Purpose

   Expression of mCherry-2xFYVE in Dictyostelium

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   2xFYVE (from human Hrs) (HGS Synthetic)
   

   Use

   Dictyostelium expression

   Tags

   mCherry

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. A3B-Cas9n-UGI-NLS

   Plasmid

   #198889

   Purpose

   Express full-length A3B BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGREAT18

   Plasmid

   #170906

   Purpose

   GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pAtUBQ10

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIII-Neo-tdTomato

   Plasmid

   #153424

   Purpose

   Retroviral (MSCV) expression of tdTomato fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   tdTomato
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

   Plasmid

   #45578

   Purpose

   Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. TetO-FUW-Sox1

   Plasmid

   #61545

   Purpose

   Expresses Sox1 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   Sox1 (Sox1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pPN217

   Plasmid

   #91676

   Purpose

   Express sgRNA targeting human TLE1

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   TLE1 (TLE1 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-TPI_PTC160-xrRNA-4H

    Plasmid

    #108369

    Purpose

    Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Mutation

    PTC160

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-TPI_WT-xrRNA-4H

    Plasmid

    #108368

    Purpose

    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

    Plasmid

    #52895

    Purpose

    Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[1]
    

    Tags

    L-IFP-F[1]

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pUC57-GSX2-tTA-BleoR

    Plasmid

    #161748

    Purpose

    Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    Advanced tTA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    endogenous GSX2 promoter

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOIMars1CyN

    Plasmid

    #75221

    Purpose

    Mammalian cell surface expression of fluorogen activating protein Mars1Cy

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    ss-HA-Mars1Cy-CD80(237-306)
    

    Tags

    HA epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLI_C-tag TNF linker mCherry

    Plasmid

    #171181

    Purpose

    inducible expression of mCherry fused to C-tag TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    C-tag TNF linker mCherry
    

    Use

    Lentiviral

    Tags

    C-tag (internal) and mCherry

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLI_BFP_C-tag TNF

    Plasmid

    #171187

    Purpose

    inducible expression of C-tag TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    C-tag TNF
    

    Use

    Lentiviral

    Tags

    C-tag (internal)

    Expression

    Mammalian

    Mutation

    ADAM cleavage site of TNF (AA73-76) was exchanged…

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMX-eSOX17WHC

    Plasmid

    #118839

    Purpose

    Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX17WHC (Sox17 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    WHC: L46W, V53H, E57C

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDT-sgRNA-XMAS-2x

    Plasmid

    #164414

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIhh742bp-Luc

    Plasmid

    #53605

    Purpose

    Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pMIII-Neo-E2Crimson

    Plasmid

    #153425

    Purpose

    Retroviral (MSCV) expression of E2-Crimson fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    E2-Crimson
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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