Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,269 results for: ef1

Clear keywords
Showing: 4481 - 4520 of 9269 results

  5. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

    Enlarge

  7. pANC61(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron-IRES-EGFP)

    Plasmid
    #248354
    Purpose
    Lentiviral vector that can express hAqp1_degron based sensor for TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP marker
    Depositor
    Arnab Mukherjee
    Article
    Chacko et al bioRxiv 2025.09.07.674790
    Insert
    FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron
    Use
    Lentiviral
    Tags
    3x TEV site, FKBP12 degron and FLAG (internal in …
    Promoter
    EF-1a
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    248354-plasmid-map-sequence-id-493085

    Enlarge

  12. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

    Enlarge

  17. E547* FL USP7

    Plasmid
    #229001
    Purpose
    E547-mutant of human USP7
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-546 (USP7 Human)
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    T7
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229001-plasmid-map-sequence-id-458328

    Enlarge

  19. JD226

    Plasmid
    #229484
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, L4-22k
    Use
    AAV and Adenoviral
    Expression
    Mammalian
    Promoter
    CMV (E2A), Ef1a (L4-22k) and wt promoter (VA RNA …
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229484-plasmid-map-sequence-id-455008

    Enlarge

  20. pDIV299

    Plasmid
    #204967
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    argB
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204967-plasmid-map-sequence-id-454123

    Enlarge

  24. pKL2-138

    Plasmid
    #219413
    Purpose
    Estradiol-inducible expression of 42-10 Zinc finger activator
    Depositor
    Ahmad Khalil
    Article
    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.
    Insert
    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
    Expression
    Yeast
    Mutation
    Four Arg to Ala mutations in zinc finger backbone
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    219413-plasmid-map-sequence-id-434474

    Enlarge

  25. pITEdv1

    Plasmid
    #218283
    Purpose
    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218283-plasmid-map-sequence-id-433685

    Enlarge

  26. pITEu2

    Plasmid
    #218282
    Purpose
    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218282-plasmid-map-sequence-id-433684

    Enlarge

  27. pJE13HD7

    Plasmid
    #218281
    Purpose
    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218281-plasmid-map-sequence-id-433683

    Enlarge

  30. pYSD101

    Plasmid
    #212925
    Purpose
    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SUC2 (SUC2 Budding Yeast)
    Expression
    Yeast
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    212925-plasmid-map-sequence-id-439532

    Enlarge

  31. pYSD140

    Plasmid
    #212927
    Purpose
    Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    mRuby2
    Expression
    Yeast
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212927-plasmid-map-sequence-id-421795

    Enlarge

  32. pYSD113

    Plasmid
    #212926
    Purpose
    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SUC2 (SUC2 Budding Yeast)
    Expression
    Yeast
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212926-plasmid-map-sequence-id-421719

    Enlarge

  38. pDIV492

    Plasmid
    #177700
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177700-plasmid-map-sequence-id-366535

    Enlarge

  39. pDIV491

    Plasmid
    #177699
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177699-plasmid-map-sequence-id-353309

    Enlarge

  40. pDIV488

    Plasmid
    #177696
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177696-plasmid-map-sequence-id-352586

    Enlarge

Showing: 4481 - 4520 of 9269 results