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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,041 results for: Ank

Showing: 5601 - 5650 of 7041 results

  5. pHREAC

    Plasmid
    #134908
    Purpose
    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    double 35S
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134908-plasmid-map-sequence-id-342556

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  9. pDIV643

    Plasmid
    #226182
    Purpose
    Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi
    Insert
    uidA marker flanked with targeting regions to KU70 gene in K.phaffii
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TEF1p
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226182-plasmid-map-sequence-id-449156

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  12. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid
    #217635
    Purpose
    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.
    Depositor
    Martin Kampmann
    Insert
    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1alpha and mU6
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217635-plasmid-map-sequence-id-449624

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  13. pAGD335

    Plasmid
    #219499
    Purpose
    pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219499-plasmid-map-sequence-id-435415

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  14. pAIDA1

    Plasmid
    #79180
    Purpose
    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags
    Depositor
    Gen Larsson
    Article
    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi
    Insert
    Adhesin Involved in Diffuse Adherence
    Use
    Tags
    His and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    79180-plasmid-map-sequence-id-170854

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  19. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

    Plasmid
    #228360
    Purpose
    hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36
    Depositor
    Michael Boettcher
    Article
    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral; Rfxcas13d grna expression …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    228360-plasmid-map-sequence-id-454335

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  20. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61737-plasmid-map-sequence-id-190162

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  24. pYC-C

    Plasmid
    #207114
    Purpose
    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus
    Depositor
    Yifan Cheng
    Article
    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207114-plasmid-map-sequence-id-414780

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  26. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Francisco Malagon, Sankar Adhya
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Use
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Promoter
    Available since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51559-plasmid-map-sequence-id-78831

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  28. pYC-N

    Plasmid
    #207113
    Purpose
    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus
    Depositor
    Yifan Cheng
    Article
    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    207113-plasmid-map-sequence-id-410641

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  30. pGWB508-cTP-sfGFP

    Plasmid
    #213863
    Purpose
    Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)
    Depositor
    Yutaka Kodama
    Article
    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.
    Insert
    Plastid transit peptide fused sfGFP
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213863-plasmid-map-sequence-id-425331

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  31. pGWB508-cTP-mCherry

    Plasmid
    #213862
    Purpose
    Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)
    Depositor
    Yutaka Kodama
    Article
    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.
    Insert
    Plastid transit peptide fused mCherry-UnaG
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213862-plasmid-map-sequence-id-425330

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  32. Halo-DNAPKcs HRD

    Plasmid
    #207088
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    207088-plasmid-map-sequence-id-470417

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  36. pHRE

    Plasmid
    #134909
    Purpose
    Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    double 35S
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134909-plasmid-map-sequence-id-267026

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  37. EasyFusion Halo-mAID

    Plasmid
    #112852
    Purpose
    General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein
    Depositor
    Janet Rossant
    Article
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Insert
    Halo-mAID
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    No
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112852-plasmid-map-sequence-id-217036

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  39. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid
    #186310
    Purpose
    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    sg61-PDGFR
    eGFP
    Use
    Tags
    His, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186310-plasmid-map-sequence-id-366939

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  40. pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

    Plasmid
    #191338
    Purpose
    Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191338-plasmid-map-sequence-id-370944

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  44. 2xMARS-nGFP

    Plasmid
    #205235
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205235-plasmid-map-sequence-id-404746

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  46. PB_TRE_sg30-PDGFR_EF1a_mCherry

    Plasmid
    #186309
    Purpose
    Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    sg30-PDGFR
    mCherry
    Use
    Tags
    His, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186309-plasmid-map-sequence-id-366940

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  49. RNF168-Halo HRD

    Plasmid
    #207080
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207080-plasmid-map-sequence-id-432323

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Showing: 5601 - 5650 of 7041 results